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CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pRRG45-GluR1

Plasmid

#99070

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea GluR1 (Glutamate receptor 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

GluR1 (Glutamate receptor 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMTH PKC epsilon

Plasmid

#8413

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_FL

Plasmid

#59391

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
hRad51(G151D)–Cas9(D10A)

Plasmid

#125565

Purpose

Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(G151D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMTH PKC eta

Plasmid

#8415

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C eta (Prkch Mouse)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pSG5HA-MAGE-KA240,245

Plasmid

#89140

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Tags

HA

Expression

Mammalian

Mutation

K240A, K245A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG260-Cyp1A1

Plasmid

#99081

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Cyp1A1 (also known as h.49.4f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Cyp1A1 (also known as h.49.4f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAdTrackCMV-PSM-Rb

Plasmid

#37107

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Adenoviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pUC 3GLA UAS HAi

Plasmid

#71763

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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hRad51(F86E)–Cas9(D10A)

Plasmid

#125564

Purpose

Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(F86E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pOT2 3GLA

Plasmid

#71764

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2H4

Plasmid

#67094

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H4 (GTF2H4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
TAL2320

Plasmid

#36746

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Left (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2321

Plasmid

#36747

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Right (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pLTR PKC epsilon

Plasmid

#8420

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
gabra5_R (TAL3015)

Plasmid

#36007

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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gabra5_L (TAL3014)

Plasmid

#36006

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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