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XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM

Plasmid

#233429

Purpose

Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-LIDARsensor(W)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP

Plasmid

#233408

Purpose

LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xPP7))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

Plasmid

#233415

Purpose

LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

Plasmid

#233439

Purpose

SFFV driven LIDAR reporter with 2 tandem substrate stem loops

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM044-3 CMV-TO-CCR7-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233395

Purpose

CCR7-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR7-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM105 CMV-TO-CX3CR1-(noV2)-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233397

Purpose

CX3CR1-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CX3CR1-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM068 CMV-TO-CX3CR1-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233398

Purpose

CX3CR1-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CX3CR1-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ113 CMV-TO-signal-hOXTR-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233399

Purpose

human oxytocin receptor-ADAR2dd(E488Q, T501A), no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

OXTR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ105 CMV-TO-signal-hOXTR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233400

Purpose

human oxytocin receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

OXTR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ142 CMV-TO-signal-B2AR-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233401

Purpose

_-2 adrenergic receptor-ADAR2dd(E488Q, T501A), no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

Plasmid

#233378

Purpose

PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ030 CMV-TO-AVPR2-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233380

Purpose

AVPR2-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and extra V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

Plasmid

#233381

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR2(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

Plasmid

#233389

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rg(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM104 CMV-TO-AVPR2-(noExtraV2)-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233390

Purpose

CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no extra V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-St1Cas9-BlastR

Plasmid

#220976

Purpose

Expresses human codon-optimized St1Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

St1Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SlugCas9-HF-BlastR

Plasmid

#220973

Purpose

Expresses human codon-optimized SlugCas9-HF

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9-HF

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge