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mTFP1-MCHR1-N-10

Plasmid

#55499

Purpose

Localization: Cilium, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MCHR1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2 S408A

Plasmid

#44109

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S408A (TRAF2 Human)

Expression

Mammalian

Mutation

Serine 408 to Alanine

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only
pWB-TRAF2 S102A

Plasmid

#44135

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Retroviral

Tags

Flag

Mutation

Serine 102 to Alanine

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S102A

Plasmid

#44106

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Expression

Mammalian

Mutation

Serine 102 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-miR-308

Plasmid

#22708

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 308 (mir-308 primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLXSN-p85α-ΔiSH2-N

Plasmid

#39502

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Mutation

ΔiSH2-N = deletion of AA478-513

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
pPS835

Plasmid

#8933

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pBABE-hygro-mTurquoise2-Nuf2

Plasmid

#80762

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pEMY01AB-PRO

Plasmid

#127714

Purpose

Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pEMY01CD-TER

Plasmid

#127751

Purpose

Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-DMVF-181-C

Plasmid

#87611

Purpose

FKBP-tagged C-terminal Dronpa fragment for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

FKBP-DMVF181-C

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS2B

Plasmid

#145354

Purpose

Cell-free expression vector for Zika virus NS2B protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Tags

mCherry and 8xHis

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS1

Plasmid

#145362

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS3

Plasmid

#145365

Purpose

Cell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has Service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLR16-Phes*

Plasmid

#98783

Purpose

Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

Depositor

Anat Herskovits

Article

Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

Insert

pheS

Expression

Bacterial

Mutation

A309G

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4)P2 Probe

Plasmid

#211511

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

TAPP1 cPHx3

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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