We narrowed to 11,687 results for: NSI
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Plasmid#165405PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT66 gene circuit
ExpressionBacterialAvailable SinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyExpressionYeastPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise-3XNLS-WPRE
Plasmid#159949Purposeoptical regulation of nuclear exportDepositorInsertmTurquoise-3XNLS
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-3XNLS-WPRE
Plasmid#159950Purposenucleus markerDepositorInsertYpet-3XNLS
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mScarlet-C1-WPRE
Plasmid#159955Purposeuntargeted markerDepositorInsertmScarlet-C1
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_Cdkn1b.a
Plasmid#107523PurposeNegative regulator of cell cycle progression by G1/S transition inhibitionDepositorInsertCdkn1b.a
PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_Cdkn1b.b
Plasmid#107524PurposeNegative regulator of cell cycle progression by G1/S transition inhibitionDepositorInsertCdkn1b.b
PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
TagsmCherryExpressionMammalianPromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19_TTE1564_UUCG-loop
Plasmid#133581PurposeT7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitchDepositorInsertP2-deletion mutant of Tte preQ1-riboswitch
UseUnspecifiedMutationreplacement of riboswitch nucleotides U6 through …PromoterT7Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xVSVG-GSK3(1-97)
Plasmid#129582PurposeEncodes VSVG tagged GSK3(1-97) for purification by GST affinityDepositorAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-3xVSVG-GSK3(1-97)
Plasmid#129583PurposeEncodes VSVG tagged GSK3(1-97) for purification by nickel affinityDepositorAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDC24
Plasmid#131368Purposeexpression of MEG-3Cterm (aa545-862) in C. elegansDepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDC21
Plasmid#131367Purposeexpression of MEG-3IDR (aa1-544) in C. elegansDepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-cI-ts_O
Plasmid#127030PurposeBasic Part O- ORF; cI; transcription factor from lambda phage; thermosensitive.DepositorInsertPart cI-ts_o
UseSynthetic BiologyExpressionBacterialMutationcI thermosensitive mutationAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-ATG13[3SD](426)
Plasmid#122077PurposeExpressing HA-ATG13[3SD]DepositorInsertATG13
Tags3HAExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SD](315)
Plasmid#122074PurposeExpressing GFP-Atg13[3SD]DepositorInsertATG13
TagsGFPExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SA](315)
Plasmid#122073PurposeExpressing GFP-Atg13[3SA]DepositorInsertATG13
TagsGFPExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Ser…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only