We narrowed to 5,034 results for: PID

1. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

   Plasmid

   #127790

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LDN

   Plasmid

   #21195

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek1 (Esr1, MAP2K1 Human, Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Mek1 K97M fused to ER*, catalytically inactive

   Promoter

   Available since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

3. FusXTBE-1397-C

   Plasmid

   #201398

   Purpose

   Expresses C terminus half of DddAtox in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   COX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FusXTBE-1397-N

   Plasmid

   #201397

   Purpose

   Expresses N terminus half of DddAtox in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   COX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE ENTR SV40-hCas9 L3-L2

   Plasmid

   #62133

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   human codon optimized Cas9
   

   Use

   CRISPR; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL-p21UTRm1

   Plasmid

   #20878

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 1 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 1 (Position ~420) GC…

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

7. mKate2-P2A-APEX2-2xML1N(7Q)

   Plasmid

   #67666

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1Nmut*2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221-TAZ CA

   Plasmid

   #79504

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   3xFLAG

   Expression

   Mutation

   S89A

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDP1.2

   Plasmid

   #171092

   Purpose

   Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
   

   Use

   Synthetic Biology

   Tags

   Emerald, Geminin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZIKV_NASBA_32B

    Plasmid

    #75011

    Purpose

    Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid

    #154838

    Purpose

    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    synthetic guide::∆HYGR::unc-54 3' UTR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    ∆HYGR is promoterless and encodes aa 60-341

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZIKV_NASBA_27B

    Plasmid

    #75010

    Purpose

    Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_27B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mApple-M1 SpastinHFDD

    Plasmid

    #134462

    Purpose

    M1 SpastinHFDD expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala at amino acid 87; His to Asp at amino …

    Promoter

    CMV

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL-p21UTRm2

    Plasmid

    #20879

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 2 (Position ~1100) G…

    Promoter

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGEMT-GDNF

    Plasmid

    #119897

    Purpose

    Mouse GDNF In situ hybridization probe

    Depositor

    Wenqin Luo

    Article

    Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

    Insert

    glial cell line derived neurotrophic factor (Gdnf Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS4 (Empty insertion vector with SEC)

    Plasmid

    #154837

    Purpose

    Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. M2E-1

    Plasmid

    #21206

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek2 (Esr1, MAP2K2 Human, Mouse)
    

    Use

    Subclone vector

    Tags

    Expression

    Mutation

    deletion of residues EQQKKRLE, S222D S226D in Mek…

    Promoter

    Available since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)

    Plasmid

    #47431

    Depositor

    Peter McPherson

    Article

    Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

    Insert

    Clavesin2 (CLVS2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pMuLE ENTR SV40-hCas9 L5-L2

    Plasmid

    #62134

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    human codon optimized Cas9
    

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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