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pTricHis-PLY-WT-no charge

Plasmid

#160539

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

K279A, Q280A, D283A, N284A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-PLY-WT-charge+

Plasmid

#160538

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

W278K, L282K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-N342W

Plasmid

#160518

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

N342W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-L340W

Plasmid

#160517

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

L340W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-N338W

Plasmid

#160516

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

N338W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pIAGO-Hap2

Plasmid

#114460

Purpose

Expression of hybrid AmphDI-PerDI (Hap) glycosyltansferase and perosamine synthase in streptomycetes

Depositor

Patrick Caffrey

Article

Hutchinson et al Chem Biol. 2010 Feb 26;17(2):174-82. doi

Insert

Hap2 + perDII

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBHDuet50K

Plasmid

#118399

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Expression

Bacterial

Mutation

incorporation of oligonucleotide during cloning

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 103-972

Plasmid

#111989

Purpose

Truncated Hsh155 (aa 103-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 103-972

Expression

Bacterial and Yeast

Mutation

103-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 237-972

Plasmid

#111992

Purpose

Truncated Hsh155 (aa 237-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 237-972

Expression

Bacterial and Yeast

Mutation

237-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 472-972

Plasmid

#111996

Purpose

Truncated Hsh155 (aa 472-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 472-972

Expression

Bacterial and Yeast

Mutation

472-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 351-972

Plasmid

#111995

Purpose

Truncated Hsh155 (aa 351-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 351-972

Expression

Bacterial and Yeast

Mutation

351-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 310-972

Plasmid

#111994

Purpose

Truncated Hsh155 (aa 310-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 310-972

Expression

Bacterial and Yeast

Mutation

310-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 275-972

Plasmid

#111993

Purpose

Truncated Hsh155 (aa 275-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 275-972

Expression

Bacterial and Yeast

Mutation

275-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 157-972

Plasmid

#111990

Purpose

Truncated Hsh155 (aa 157-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 157-972

Expression

Bacterial and Yeast

Mutation

157-972 truncation

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 197-972

Plasmid

#111991

Purpose

Truncated Hsh155 (aa 197-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 197-972

Expression

Bacterial and Yeast

Mutation

197-972 truncation

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal DsRedEx2

Plasmid

#104024

Purpose

Plasmid encoding 2ndVal DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal DsRed-Express2

Use

Low copy

Expression

Bacterial

Mutation

DsRed-Express2 with valine inserted in 2nd positi…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJK593

Plasmid

#71587

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK594

Plasmid

#71588

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with C-terminal His6 tag (YfdEH6)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Cow 1-82)

Plasmid

#62062

Purpose

Expression of Cow p53 (residues 1-82) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-82

Promoter

T7

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only