We narrowed to 6,927 results for: 11

1. pMBA337

   Plasmid

   #214750

   Purpose

   Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnEA-NpG-HsXRN1_1650-1706_P

   Plasmid

   #147219

   Purpose

   Bacterial Expression of HsXRN1_1650-1706

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1650-1706 (XRN1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-EGFP-C1-HsXRN1_1675-1706_P

   Plasmid

   #147222

   Purpose

   Mammalian Expression of HsXRN1_1675-1706

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1675-1706 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLNHA-C1-HsXRN1_1174-1342_K

   Plasmid

   #146778

   Purpose

   Mammalian Expression of HsXRN1_1174-1342

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1174-1342 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLNHA-C1-HsXRN1_1174-1458_K

   Plasmid

   #146779

   Purpose

   Mammalian Expression of HsXRN1_1174-1458

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1174-1458 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLNHA-C1-HsXRN1_1174-1706_K

   Plasmid

   #146780

   Purpose

   Mammalian Expression of HsXRN1_1174-1706

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1174-1706 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLNHA-C1-HsXRN1_1343-1534_K

   Plasmid

   #146781

   Purpose

   Mammalian Expression of HsXRN1_1343-1534

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1343-1534 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-EGFP-C1-HsXRN1_1174-1342_K

   Plasmid

   #146786

   Purpose

   Mammalian Expression of HsXRN1_1174-1342

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1174-1342 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-EGFP-C1-HsXRN1_1174-1458_K

   Plasmid

   #146787

   Purpose

   Mammalian Expression of HsXRN1_1174-1458

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1_1174-1458 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsXRN1_1343-1534_K

    Plasmid

    #146789

    Purpose

    Mammalian Expression of HsXRN1_1343-1534

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1_1343-1534 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HsXRN1_1459-1706_K

    Plasmid

    #146790

    Purpose

    Mammalian Expression of HsXRN1_1459-1706

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1_1459-1706 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmNanos_50-236_AC

    Plasmid

    #148457

    Purpose

    Insect Expression of DmNanos_50-236

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_50-236 (nos Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-EGFP-DmNanos_297-381_AC

    Plasmid

    #148458

    Purpose

    Insect Expression of DmNanos_297-381

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_297-381 (nos Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-GST-HsNanos2_6-22-DmNanos_297-381_AC

    Plasmid

    #148472

    Purpose

    Insect Expression of HsNanos2_6-22-DmNanos_297-381

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    HsNanos2_6-22-DmNanos_297-381 (NANOS2 Human, Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-GST-HsNanos2_6-22-F6AW9E-DmNanos_297-381_AC

    Plasmid

    #148473

    Purpose

    Insect Expression of HsNanos2_6-22-F6AW9E-DmNanos_297-381

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    HsNanos2_6-22-F6AW9E-DmNanos_297-381 (NANOS2 Human, Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1C-Fluc-DmHunchback-delBoxA_AD

    Plasmid

    #148501

    Purpose

    Insect Expression of DmHunchback-delBoxA

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmHunchback-delBoxA (hb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1C-Fluc-DmHunchback-delBoxB_AD

    Plasmid

    #148502

    Purpose

    Insect Expression of DmHunchback-delBoxB

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmHunchback-delBoxB (hb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnEA-NpG-DmNanos_116-163_AB

    Plasmid

    #148327

    Purpose

    Bacterial Expression of DmNanos_116-163

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_116-163 (nos Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1C-Fluc-DmHunchback_W

    Plasmid

    #147818

    Purpose

    Insect Expression of DmHunchback-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmHunchback-3UTR (hb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b I-SceI (Ctermhis) K223I

    Plasmid

    #195615

    Purpose

    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C2694G, G2345C, G2351C, A3365T, A3366T

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET15b I-SceI (Ctermhis) K122I

    Plasmid

    #195614

    Purpose

    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C2458G, A2826T, A2827T

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-CBS (H65R)

    Plasmid

    #182924

    Purpose

    Mammalian expression plasmid for human CBS mutant H65R

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed histidine 65 to arginine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKIF1C_P176L-mCherry-FRB

    Plasmid

    #185983

    Purpose

    Mammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    mCherry and FRB

    Expression

    Mammalian

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. OfCasp3a

    Plasmid

    #166053

    Purpose

    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Orbicella faveolata caspase-3a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PaCasp7a

    Plasmid

    #166051

    Purpose

    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Porites astreiodes caspase-7a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AlkB-D135G

    Plasmid

    #160434

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Glycine; deleted the…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22b-ecDHFR-dCys

    Plasmid

    #110541

    Purpose

    Bacterial protein of E.coli DHFR C85A/C152S mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C85 mutated to A85, C152 mutated to S152

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge