We narrowed to 7,286 results for: 11

1. pCfB3039(XII-2 MarkerFree)

   Plasmid

   #73279

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3_020

   Plasmid

   #123735

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   ERT2-fkbp-spdCa9(205-1368)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR4-Dact1(1250-1601)

   Plasmid

   #52046

   Purpose

   To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

   Depositor

   Ben Cheyette

   Article

   Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

   Insert

   Dact1(1250-1601) (Dact1 Mouse)
   

   Expression

   Bacterial

   Available Since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLV-shGrm2-2344

   Plasmid

   #120725

   Purpose

   Lentiviral vector that expresses GFP and an shRNA targeting Grm2 (in pLL3.7)

   Depositor

   Amy Lasek

   Article

   Ding et al Alcohol Clin Exp Res. 2017 Nov;41(11):18

   Insert

   Grm2 (Grm2 Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-shGrm2-972

   Plasmid

   #120724

   Purpose

   Lentiviral vector that expresses GFP and an shRNA targeting Grm2 (in pLL3.7)

   Depositor

   Amy Lasek

   Article

   Ding et al Alcohol Clin Exp Res. 2017 Nov;41(11):18

   Insert

   Grm2 (Grm2 Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-TFAP4(E135A/S139A)

   Plasmid

   #110157

   Purpose

   Expression of human TFAP4 (E135A/S139A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E135A/S139A

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-His6-Ubc9-C93A

   Plasmid

   #133910

   Purpose

   Bacterial expression of His6-Ubc9-C93A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 93 to Alanine

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB3036(XI-1 MarkerFree)

   Plasmid

   #73274

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Myc-PSK2

    Plasmid

    #197117

    Purpose

    Expression of Myc-tagged PSK2 / TAOK1 in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

    Insert

    TAOK1 (TAOK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    SV40

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK0_043

    Plasmid

    #123943

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - A1 Insulator - HygroR
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaG-24

    Plasmid

    #172750

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-24
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sha2Cas9-HF

    Plasmid

    #192142

    Purpose

    Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Sha2Cas9-HF (R247A and N415A)

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3542 pMIG Bcl-XL mut1

    Plasmid

    #8791

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    F131V, D133A

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTK3_001

    Plasmid

    #123952

    Purpose

    Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3XNLS::CRY2PHR::p65
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cs2+Etv5

    Plasmid

    #49095

    Purpose

    expression of Etv5

    Depositor

    Michael Tsang

    Article

    Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

    Insert

    Etv5 (etv5a Zebrafish)
    

    Use

    Xenopus/avian/zebrafish

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-His6-Ubc9-C138A

    Plasmid

    #133911

    Purpose

    Bacterial expression of His6-Ubc9-C138A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 138 to Alanine

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge