Addgene

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ABE8e

Plasmid

#138489

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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SPDK3959

Plasmid

#199246

Purpose

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

Insert

Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

Promoter

PEBV

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM47523

Plasmid

#153221

Purpose

Level 0 zCas9i vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

SpCas9 with introns and 2 NLS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PE-bacteria

Plasmid

#172715

Purpose

Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

Cas9n-linker-M-MLV2

Use

Tags

Expression

Bacterial

Mutation

H840A of Cas9

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCJ1021

Plasmid

#228761

Purpose

Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 gRNAs (Ift88 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Article

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Expression

Mutation

Promoter

MSCV

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJRH-1187 VSVGmut

Plasmid

#201913

Purpose

Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

VSV-G (K47Q, R354A)

Use

Tags

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHL-sec

Plasmid

#99845

Purpose

Mammalian expression with secretion signal sequence

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EFNB2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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XLone-Cas9-2A-GFP

Plasmid

#170159

Purpose

Express Cas9 and GFP upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

Cas9

Use

Synthetic Biology; Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE3G promoter

Available since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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#1.4 post-G-SynapShot-mScarlet

Plasmid

#214716

Purpose

Expresses green #1.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-G-SynapShot

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Blasto

Plasmid

#167856

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-2

Plasmid

#61737

Purpose

Streptomyces expression of codon-optimized Cas9 and custom gRNA

Depositor

Huimin Zhao

Article

Cobb et al ACS Synth Biol. 2014 Dec 8.

Inserts

sSpCas9
gRNA cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

BbsI-flanked lacZ cassette inserted in place of s…

Promoter

gapdhp(EL) and rpsL(XC)-BbsI

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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PE-P3-rice

Plasmid

#185612

Purpose

For plant prime editing in rice plants

Depositor

Jinxiao Yang

Article

Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

Insert

3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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