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SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFP

Plasmid

#244206

Purpose

Mammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastR

Plasmid

#244207

Purpose

Mammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

Plasmid

#244216

Purpose

Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS

Plasmid

#241843

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

VP16

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

VP16 minimal activation domain x 3

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE2.5

Plasmid

#235054

Purpose

Expresses Tad-CABE2.5 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE2.5-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.1

Plasmid

#235056

Purpose

Expresses Tad-CBE3.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.2

Plasmid

#235057

Purpose

Expresses Tad-CBE3.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.1

Plasmid

#235051

Purpose

Expresses Tad-CABE1.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.2

Plasmid

#235052

Purpose

Expresses Tad-CABE1.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CT751

Plasmid

#233657

Purpose

Dox-inducible VP64-MyoD

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

VP64-MyoD

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT754

Plasmid

#233659

Purpose

FLEx switch with tTA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

tTA FLEx switch

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMO819

Plasmid

#232072

Purpose

Expresses Cytosine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Cytosine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMO1170

Plasmid

#232073

Purpose

Expresses Adenine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Adenine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSCD-GSDMA-C

Plasmid

#226235

Purpose

Express GSDMA-C domain

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Gasdermin A (Gsdma3 Mouse)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-cTEVP-RBD

Plasmid

#226233

Purpose

Express cTEVP-RBD fusion

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

cTEVP-RBD (TEVgp1 Tobacco etch virus)

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-nTEVP-RBD

Plasmid

#226232

Purpose

Express nTEVP-RBD fusion

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

nTEVP-RBD (TEVgp1 Tobacco etch virus)

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-HCVP

Plasmid

#226227

Purpose

Express HCVP

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

HCVP (POLY Hepatitis C virus)

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-pA

Plasmid

#210584

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-U

Plasmid

#217003

Purpose

Expression of Small BiT-Linker-GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpU

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-G-Sm

Plasmid

#216990

Purpose

Expression of GvpG-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpG-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-T-Sm

Plasmid

#217000

Purpose

Expression of GvpT-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpT-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-J

Plasmid

#216999

Purpose

Expression of Small BiT-Linker-GvpJ

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpJ

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-U-Sm

Plasmid

#217002

Purpose

Expression of GvpU-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpU-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-S

Plasmid

#216995

Purpose

Expression of Small BiT-Linker-GvpS

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpS

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-L-Sm

Plasmid

#216992

Purpose

Expression of GvpL-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpL-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Sm-N

Plasmid

#216987

Purpose

Expression of Small BiT-Linker-GvpN

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpN

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-N-Sm

Plasmid

#216986

Purpose

Expression of GvpN-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpN-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-R-Sm

Plasmid

#216984

Purpose

Expression of GvpR-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpR-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Sm-B

Plasmid

#216983

Purpose

Expression of Small BiT-Linker-GvpB

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpB

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge