We narrowed to 13,673 results for: TIM

1. RPS6KA3 gRNA (BRDN0001144943)

   Plasmid

   #75942

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

   Plasmid

   #174630

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   GFP-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RPS6KA3 gRNA (BRDN0001148452)

   Plasmid

   #75943

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SSpB-mCherry-p63DH

   Plasmid

   #176112

   Purpose

   Heterodimerization with iLID, RhoA activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   RHOGEF p63 (ARHGEF25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TO-Nprot-HF_GC3opt

   Plasmid

   #157732

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nprot-HF_GC3opt (N SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

   Plasmid

   #188769

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166010

   Purpose

   mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S )
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UbC-tdTomato

   Plasmid

   #62516

   Purpose

   Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures

   Depositor

   Pantelis Tsoulfas, Jae Lee

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Insert

   tdTomato
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mARGAna (cassette 2)

    Plasmid

    #191342

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPPI4-Strep-SNAP-CTLA-4-His6

    Plasmid

    #223583

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCG_batCoV RaTG13 N C-V5-tag

    Plasmid

    #179987

    Purpose

    Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    RaTG13 N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG_Xph15-eGFP-CCR5TC

    Plasmid

    #135528

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188772

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid

    #140000

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

    Plasmid

    #166011

    Purpose

    mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV51

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CSNK1G2 gRNA (BRDN0001162501)

    Plasmid

    #76988

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSNK1G2 (CSNK1G2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CSNK1G2 gRNA (BRDN0001149101)

    Plasmid

    #76989

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSNK1G2 (CSNK1G2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CSNK1G2 gRNA (BRDN0001145026)

    Plasmid

    #76990

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSNK1G2 (CSNK1G2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RPS6KA3 gRNA (BRDN0001147852)

    Plasmid

    #75944

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA3 (RPS6KA3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid

    #206259

    Purpose

    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    GTS mTagBFP
    

    Use

    Multimate/gateway entr 4

    Tags

    BFGT1 (Golgi localisation peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-45b-HP1a-mCherry

    Plasmid

    #185012

    Purpose

    Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    CBX5 (HP1a) (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac (GST-SNAP-Shp2)

    Plasmid

    #177897

    Purpose

    Baculo expression of GST tag fused to SNAP tag and human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-DDX11-Flag-WT

    Plasmid

    #120727

    Purpose

    Expression of a Flag-tagged version of DDX11 WT in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 (DDX11 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mARGAna (cassette 2, transient)

    Plasmid

    #197589

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMMACE DEST CMV H2B iRFP

    Plasmid

    #206253

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713
    

    Use

    Recombinant baculovirus production, multimate/gat…

    Tags

    iRFP713

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAuroraA GFP-AURKA-GFP

    Plasmid

    #157765

    Purpose

    Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    promoter CMV replaced by AurkA promoter

    Promoter

    AURKA minimal

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SSpB-iRFP-p63DH

    Plasmid

    #176120

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid

    #174631

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge