We narrowed to 6,668 results for: PROC

1. pET41sIII_GST-ALC1-K77R

   Plasmid

   #174813

   Purpose

   Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-K77R (CHD1L Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K77R

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41sIII_GST-ALC1-E175Q

   Plasmid

   #174814

   Purpose

   Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-E175Q (CHD1L Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E175Q

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

   Plasmid

   #135563

   Purpose

   An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   shRNA (scrambled)
   Nuc-EYFP
   

   Use

   AAV and RNAi

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CMV-IE and mU6

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. deltaLoop-MIDAS

   Plasmid

   #171605

   Purpose

   Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
   

   Tags

   His6 tag and SNAP tag

   Expression

   Bacterial

   Mutation

   Replaced amino acids 4458-4496 with ASGSGS linker

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer10-UBC9-sh3

   Plasmid

   #168987

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Expression

   Mammalian

   Promoter

   Ubc promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHis-Hgd

   Plasmid

   #163091

   Purpose

   Expressing mouse Hgd in bacterial cells

   Depositor

   Joseph Takahashi

   Article

   Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

   Insert

   Hgd (Hgd Mouse)
   

   Tags

   his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. QuasAr-P2A-hKvb1

   Plasmid

   #154083

   Purpose

   Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

   Depositor

   Michael Hoppa

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

   Insert

   QuasAr-P2A-human Kvb1 (KCNAB1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   myc-Flag

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3/flag-pBirA(1-K283)-FRB

   Plasmid

   #154451

   Purpose

   expresses flag-pBirA(1-K283)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-K283)-FRB
   

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-XYLT2-sgRNA

   Plasmid

   #154862

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting XYLT2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   XYLT2 sgRNA (XYLT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-H2-Ld-VN

    Plasmid

    #135520

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. hsa-cdk2 3'UTR sicheck2 mut

    Plasmid

    #121124

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
    

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-lyzC-mcherry-2a-CDK2-D145N

    Plasmid

    #121132

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-D145N CDK2 DN (cdk2 Zebrafish)
    

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    D145N point mutation DN

    Promoter

    neutrophil specific lyzC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. dre-cdk2 3'UTR sicheck2 199 mut

    Plasmid

    #121122

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
    

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. dre-cdk2 3'UTR sicheck2

    Plasmid

    #121123

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-cdk2 3'UTR (cdk2 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid

    #118269

    Purpose

    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

    Promoter

    T7 Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a-VHH-1xTS

    Plasmid

    #109418

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMGF169

    Plasmid

    #96996

    Purpose

    ish1 mCherry insertion cassette with G418 resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + G418 …

    Promoter

    N/A

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid

    #80638

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 217 to Alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid

    #80637

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Trp 220 to Ala

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid

    #80639

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 214 to Alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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