We narrowed to 9,825 results for: 158

1. pCWXPGR-pTF-DVL2deltaDIX

   Plasmid

   #114276

   Purpose

   TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   DVL2 (Dvl2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of DIX domain

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-MRPP3-His

   Plasmid

   #67866

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

   Insert

   MRPP3 (KIAA0391 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO GFP MPS1 K-R

   Plasmid

   #59819

   Purpose

   Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

   Insert

   Mps1 (TTK Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   KD, catalytically inactive, D664A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV4Neo-murine IL-17RASEFIR-HA

   Plasmid

   #46863

   Purpose

   expresses mouse IL-17RA with SEFIR deletion

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA-delta-SEFIR (Il17ra Mouse)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Deletion of SEFIR domain (AA 394-485)

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

   Plasmid

   #163571

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CD8a-hnRNP-M-EGFP

   Plasmid

   #86054

   Purpose

   A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CD8a luminal and transmembrane domians along with…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

   Plasmid

   #74263

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.R404X truncation

   Promoter

   CMV

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

   Plasmid

   #119319

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP III (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 1-84

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-DTYMK

   Plasmid

   #100546

   Purpose

   mammalian expression of deoxythymidylate kinase

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

   Insert

   deoxythymidylate kinase (DTYMK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX028

    Plasmid

    #167151

    Purpose

    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnHispS-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5 beta4 1355T

    Plasmid

    #16038

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pIG-707pUC19-tNGFR-P2A-TTC37

    Plasmid

    #186122

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    TTC37 (SKIC3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV_RBMX-eA3A-UdgX-NG-nCas9

    Plasmid

    #163551

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX018

    Plasmid

    #167144

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal luciferase, nnLuz
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CD8-dCherry-FRB

    Plasmid

    #100739

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and dCherry

    Expression

    Mammalian

    Mutation

    Dark mCherry (K70N) non-fluorescent

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. plenti-UbcP-FLAG-YWAA-pGK-HYG

    Plasmid

    #107375

    Purpose

    Lentiviral expression of FLAG-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX PLCg2(NC)-SH2

    Plasmid

    #46472

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg2(NC) (PLCG2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 524-749

    Promoter

    Tac

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

    Plasmid

    #181842

    Purpose

    EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only residues 12-112 of PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX029

    Plasmid

    #167152

    Purpose

    MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnH3H-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge