We narrowed to 4,189 results for: Pet2

1. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3I91

   Plasmid

   #62514

   Purpose

   Bacterial expression plasmid for use in structural determination.

   Depositor

   Cheryl Arrowsmith

   Article

   Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

   Insert

   CBX8 (CBX8 Human)
   

   Use

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pBJG1 T7-8xHis-OGT 5N5A

   Plasmid

   #154280

   Purpose

   Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; bact…

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His6tev CBP BCHZDeltaAL

   Plasmid

   #99551

   Purpose

   Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   replaced 1557-1618 with SGGSG

   Promoter

   T7

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pdpy-30-miniCAFE

   Plasmid

   #170115

   Purpose

   Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   Dpy-30

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His6-SMT3-SopB

   Plasmid

   #183676

   Purpose

   Recombinant Salmonella Typhimurium SopB

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSN672

   Plasmid

   #177362

   Purpose

   Expresses human KIF1A(1-393) fused with leucine zipper and His tag

   Depositor

   Shinsuke Niwa

   Article

   Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

   Insert

   KIF1A (KIF1A Human)
   

   Use

   Tags

   His tag and Leucine zipper

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His-GINA3

   Plasmid

   #74980

   Purpose

   Expresses His tagged GINA3 protein in bacteria.

   Depositor

   Pradipta Ghosh

   Article

   Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

   Insert

   Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. TTR C10S S85C F87E

    Plasmid

    #238074

    Purpose

    Bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S S85C F87E

    Promoter

    T7

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ce iPGM-C-HiBiT

    Plasmid

    #207120

    Purpose

    Bacterially expressed iPGM-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    

    Use

    Tags

    HiBiT Tag and Thrombin cleavage site + 10x His Ta…

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E. coli Lig-N-HiBiT

    Plasmid

    #215437

    Purpose

    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    

    Use

    Tags

    10xHis-tag with thrombin site and HiBiT Tag

    Expression

    Bacterial

    Mutation

    Codon Optimized for bacterial expression, T7 gene…

    Promoter

    T7 Promotor

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. E. coli Lig-C-HiBiT

    Plasmid

    #215438

    Purpose

    Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
    

    Use

    Tags

    10xHis-tag with thrombin site and HiBiT Tag

    Expression

    Bacterial

    Mutation

    Codon Optimized for bacterial expression, T7 gene…

    Promoter

    T7 Promotor

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINQ-ON10

    Plasmid

    #198689

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, HA tag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pINQ-NbH4

    Plasmid

    #198690

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody H4
    

    Use

    Tags

    6xHis tag, Avitag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hzHDAC10

    Plasmid

    #225542

    Purpose

    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

    Insert

    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Mutated A24E and D94A

    Promoter

    T7

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H6Gb1-CREB(1-280) C90A, T172C

    Plasmid

    #217983

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    C90A, T172C

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge