We narrowed to 4,353 results for: erf

1. pSUMO R106L-CPTP

   Plasmid

   #170737

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   R106L-CPTP (CPTP Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   R106L

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO K60A-CPTP

   Plasmid

   #170736

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   K60A-CPTP (CPTP Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   K60A

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

   Plasmid

   #166805

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   LL193EE

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

   Plasmid

   #166808

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K1277E

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

   Plasmid

   #166807

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H1276G

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R

   Plasmid

   #166806

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   P1214R

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB.His.MBP.3C NLRP1 UPA-CARD E1461R

   Plasmid

   #164007

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   E1461R

   Promoter

   Available Since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB.His.MBP.3C NLRP1 UPA-CARD W1460A

   Plasmid

   #164006

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   W1460A

   Promoter

   Available Since

   Feb. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB.His.MBP.3C NLRP1 UPA-CARD M1457A

   Plasmid

   #164005

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   M1457A

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A

    Plasmid

    #164004

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Y1445A

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A

    Plasmid

    #163989

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    W1460A

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

    Plasmid

    #163987

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y1445A

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTMB352_ZFcharm_Kv1_split-inteins

    Plasmid

    #220848

    Purpose

    Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv2
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRIPZ-bioGEF-UBwt-PURO

    Plasmid

    #208045

    Purpose

    Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    Ub (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL21-VEX

    Plasmid

    #158230

    Purpose

    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

    Depositor

    Junwei Shi

    Article

    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-IRF4

    Plasmid

    #58987

    Purpose

    MSCV based expression vector - co-expresses GFP and murine IRF4

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Insert

    Mouse IRF4 (Irf4 Mouse)
    

    Use

    Retroviral

    Tags

    Kozak sequence added

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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