We narrowed to 18,395 results for: tec

1. nls-EGFP

   Plasmid

   #67652

   Purpose

   Mammalian expression vector driving nls-EGFP.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   nls-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIMcpf1

   Plasmid

   #153034

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1328 LV EF1a-CD20 IRES-EGFP

   Plasmid

   #201918

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD20
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9 TLS2

   Plasmid

   #196978

   Purpose

   Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS2 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9 TLS1

   Plasmid

   #196977

   Purpose

   Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS1 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p201N Cas9

   Plasmid

   #59175

   Purpose

   Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   nptII
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. COM-epegRNA-Dnmt1

   Plasmid

   #211376

   Purpose

   Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   COM-epegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EK0396 BFPmut-stop-t70587 (pEAQ)

    Plasmid

    #191171

    Purpose

    EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t70587 (plant)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-TurboID-NES_pCDNA3

    Plasmid

    #107169

    Purpose

    expresses V5-tagged TurboID in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN7HELIX

    Plasmid

    #190662

    Purpose

    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ABE8e

    Plasmid

    #138489

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pS2513·PHP

    Plasmid

    #122590

    Purpose

    Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

    Depositor

    Pablo Ivan Nikel

    Article

    Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

    Insert

    phIorine2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pem7

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-N1

    Plasmid

    #54642

    Purpose

    Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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