We narrowed to 6,469 results for: poly

1. pcDNA5-FRT-TO-His6-NUbo-H2B-His8

   Plasmid

   #212811

   Purpose

   Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   H2B
   

   Use

   Tags

   6xHis-NUbo and 8xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-His8-H2B-CUbo

   Plasmid

   #212812

   Purpose

   Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   H2B
   

   Use

   Tags

   8xHis and CUbo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

   Plasmid

   #212813

   Purpose

   Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   H2B
   

   Use

   Tags

   8xHis and CUbo(K48R)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SUMO-Twinkle 360-684

   Plasmid

   #205055

   Purpose

   TWNK protein expression

   Depositor

   Smita Patel

   Article

   Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

   Insert

   TWNK (TWNK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks N terminal amino acid from 1-359

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_V222A RAD23B

   Plasmid

   #201580

   Purpose

   Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation V222A

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-AN-DDK_F400A RAD23B

   Plasmid

   #201581

   Purpose

   Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation F400A

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-IEo

   Plasmid

   #201992

   Purpose

   AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

   Depositor

   Wei Xu

   Article

   Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

   Insert

   Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Tight TRE

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. DsRed -7e

   Plasmid

   #191765

   Purpose

   Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of negatively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. H1.M10.luc

   Plasmid

   #193706

   Purpose

   Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV.CBA.YFP.miR-E_shSrpk3-1

    Plasmid

    #180400

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-1 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV.CBA.YFP.miR-E_shSrpk3-2

    Plasmid

    #180401

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-2 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CBA.YFP.miR-E_shSrpk3-3

    Plasmid

    #180402

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-3 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST_YlHD (pBS0275)

    Plasmid

    #185153

    Purpose

    For the mammalian expression of the Yarrowia fungus protein YlHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    YlHD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDS12_Cas9-NLS

    Plasmid

    #136122

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_AtcoCas9-NLS(noStop)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNG1304

    Plasmid

    #162488

    Purpose

    Expresses HelD K239A from Bacillus subtillis

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    HelD (helD Bacillus subtillis 168)
    

    Use

    Tags

    6His-V5

    Expression

    Bacterial

    Mutation

    Changed Lysine 239 to Alanine (K239A)

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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