We narrowed to 10,863 results for: HA

1. pOZ-C 16 E7

   Plasmid

   #13705

   Depositor

   Karl Munger

   Article

   Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

   Insert

   HPV 16 E7 (E7 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pUb dRxCas13d + RxCas13d guide RNA

   Plasmid

   #176304

   Purpose

   Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TAL-L1-2.4-VP64

   Plasmid

   #103039

   Purpose

   Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-2.4-VP64
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   HA and VP64

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOZ-N 16 E7

   Plasmid

   #13706

   Depositor

   Karl Munger

   Article

   Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

   Insert

   HPV 16 E7 (E7 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-nDsRedFL-miR9/9*

   Plasmid

   #177805

   Purpose

   The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-9/9*
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS Wnt-3aHA

   Plasmid

   #17374

   Depositor

   Jan Kitajewski

   Article

   Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

   Insert

   Wnt-3aHA (Wnt3a Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 7, 2008

   Availability

   Academic Institutions and Nonprofits only

8. TAL-L1-1.3-KRAB

   Plasmid

   #103034

   Purpose

   [Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-1.3-KRAB
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   HA and KRAB

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 NHE-RF

   Plasmid

   #11635

   Depositor

   Vijaya Ramesh

   Article

   Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

   Insert

   NHE-RF (NHERF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pUB6-VSV-GS

    Plasmid

    #190781

    Purpose

    Expresses VSV-GS in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    VSV-GS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICH-nuc-roGFP2-GPXC71S

    Plasmid

    #191760

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    GPX has no peroxidatic Cysteine 71, which is repl…

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pClneoHA mouse Numb

    Plasmid

    #37012

    Depositor

    Yutaka Hata

    Article

    Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

    Insert

    Numb (Numb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains Numb aa 8-853

    Promoter

    CMV

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

    Plasmid

    #31480

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (ani target)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S, M16L to reduce background mCherr…

    Promoter

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA

    Plasmid

    #135462

    Purpose

    Neuronal expression of uFLARE TF component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
    

    Use

    AAV

    Tags

    HA, flag, VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4TO OM-eLIR

    Plasmid

    #189004

    Purpose

    mitophagy-inducing adaptor protein

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    OM-eLIR
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV LDTS-LIRmut

    Plasmid

    #189001

    Purpose

    lipophagy-inducing adaptor protein, negative control

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-LIRmut
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Tags

    HA and NLS

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-nDsRedFL-miR124

    Plasmid

    #177806

    Purpose

    The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TAL-L1-2.4-Ctrl

    Plasmid

    #103037

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-2.4
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJH4531

    Plasmid

    #132366

    Purpose

    Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-2 (daf-2 Nematode)
    

    Use

    Tags

    none

    Expression

    Worm

    Mutation

    Promoter

    Prgef-1

    Available Since

    Sept. 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGBW-m4046758

    Plasmid

    #145670

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. 3xFRB-myc

    Plasmid

    #20226

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pNOC-CRISPR

    Plasmid

    #100008

    Purpose

    Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BII-ChPtW-PLAGL2

    Plasmid

    #133380

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    PLAGL2 (PLAGL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-nDsRedFL-miRNT

    Plasmid

    #177808

    Purpose

    A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-NT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHA-ΔN335-Kin1A

    Plasmid

    #31606

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN335 kinesin-1A (Kif5a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-335

    Promoter

    CMV

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

27. TAL-L1-Tf1-Ctrl

    Plasmid

    #103036

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-Tf1
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1 AT1R DRY-AAY

    Plasmid

    #45759

    Depositor

    Robert Lefkowitz, Laszlo Hunyady

    Article

    Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

    Insert

    AT1R DRY-AAY (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed amino acids 125-126 from DR to AA

    Promoter

    CMV

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pShGSK3bE

    Plasmid

    #37526

    Depositor

    Jerry Crabtree

    Article

    Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

    Insert

    GSK3b (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SRa promoter

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mouse Cavin-1

    Plasmid

    #50441

    Purpose

    expresses mouse Cavin-1 in mammalian cells

    Depositor

    Paul Pilch

    Article

    Liu et al J Biol Chem. 2008 Feb 15;283(7):4314-22.

    Insert

    Cavin-1 (Cavin1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pLPC-FLAG.HA.HA.Mm.RAP1

    Plasmid

    #184642

    Purpose

    Retroviral expression of mouse RAP1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    RAP1
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pEGFP-Akt(R25C)

    Plasmid

    #39532

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt(R25C) (Akt1 Mouse)
    

    Use

    Tags

    6G linker, EGFP, and HA

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pGL3-Control-miR-20-Control

    Plasmid

    #21169

    Depositor

    Joshua Mendell

    Article

    O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

    Insert

    miR-20 Sensor (MIR20A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sensor construct insert has two miR-20 binding si…

    Promoter

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

    Plasmid

    #81206

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 3158 pSFFV-neo Bad cDNA

    Plasmid

    #8753

    Depositor

    Stanley Korsmeyer

    Article

    Yang et al Cell 1995 Jan 27;80(2):285-91.

    Insert

    BAD (Bad Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

39. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBRIT Pax7 216C

    Plasmid

    #17523

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-215

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only