We narrowed to 8,902 results for: BLI;

1. loxP-T2A-Myr-DeadCasp8-ERT2-rox

   Plasmid

   #182361

   Purpose

   Shuttle vector for T2A-Myr-DeadCasp8-ERT2

   Depositor

   Mario Capecchi

   Insert

   loxP-T2A-Myr-DeadCasp8-ERT2-rox
   

   Use

   Cre/Lox and Mouse Targeting; Dre / rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSP1D12W/2Y

   Plasmid

   #124275

   Purpose

   Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence

   Depositor

   Alexey S. Ladokhin

   Insert

   Membrane scaffold protein
   

   Use

   Tags

   7xHis-tag

   Expression

   Bacterial

   Mutation

   Two tryptophans changed to two tyrosines

   Promoter

   T7

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDZ2FE_Rev3_1873-2025

   Plasmid

   #105672

   Purpose

   Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein

   Depositor

   Thanos Halazonetis

   Insert

   Rev3L (REV3L Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-EOLA1/CXORF40A

   Plasmid

   #180776

   Purpose

   Expresses EOLA1/CXORF40A in mammalian cells

   Depositor

   Douglas Feldman

   Insert

   EOLA1 (EOLA1 Human)
   

   Use

   Tags

   SBP-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SDCBPA-c020

   Plasmid

   #175484

   Purpose

   Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.

   Depositor

   Opher Gileadi

   Insert

   SDCBP (SDCBP Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. STX4AA-c005

   Plasmid

   #175487

   Purpose

   Protein expression in bacterial cells. Coiled-coil domains, M1-L275.

   Depositor

   Opher Gileadi

   Insert

   STX4 (RUNX1T1 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

   Plasmid

   #172477

   Purpose

   sfGFP with a synthetic intron derived from the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

   Plasmid

   #172478

   Purpose

   sfGFP with a synthetic intron from the pCI expression vector (Promega)

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

   Plasmid

   #172475

   Purpose

   sfGFP with a synthetic intron derived from the mouse Ighe gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

    Plasmid

    #172476

    Purpose

    sfGFP with a synthetic intron derived from the rabbit beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRNATU6.1 HDAC3 shRNA1

    Plasmid

    #112249

    Purpose

    HDAC3 KD

    Depositor

    Simone Di Giovanni

    Insert

    shRNA against mouse HDAC3 (Hdac3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pANY4-pR18/pL18-ccdB

    Plasmid

    #164899

    Purpose

    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.

    Depositor

    Yingfeng An

    Insert

    ccdB cassette
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL1271

    Plasmid

    #129282

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)

    Depositor

    Lee Lynd

    Insert

    alpha-Xylp_1211 (Ga0256695_ 1211)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KSP_HNL

    Plasmid

    #129772

    Purpose

    Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

    Depositor

    William Hancock

    Insert

    KSP (kif11.L Frog)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 367

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Unc104_K560GFP

    Plasmid

    #129773

    Purpose

    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

    Depositor

    William Hancock

    Insert

    Unc104 (unc-104 Nematode)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 361, fused to kinesin-1 starting Al…

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-mTRAF3ip3∆TM-CFP

    Plasmid

    #125182

    Purpose

    Expresses mouse TRAF3IP3 ∆TM in mammalian cells

    Depositor

    Shao-Cong Sun

    Insert

    TRAF3ip3 (Traf3ip3 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    K87R and S359P- See depositor comments below

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR_Hs_MBD3_ex2

    Plasmid

    #62623

    Purpose

    Encodes gRNA which targets human MBD3

    Depositor

    Paul Wade

    Insert

    MBD3 gRNA (MBD3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

19. LentiCRISPR_Hs_MBD3_ex3

    Plasmid

    #62624

    Purpose

    Encodes gRNA which targets human MBD3

    Depositor

    Paul Wade

    Insert

    MBD3 gRNA (MBD3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pmSod1-Q42L;Q123A-N’His

    Plasmid

    #117710

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Use

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Q42L; Q123A

    Promoter

    T7

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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