We narrowed to 7,271 results for: Tet-on

1. pKA-207I10

   Plasmid

   #79845

   Purpose

   Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
   

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVS2

   Plasmid

   #33144

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Inserts

   LacO (256 copies)
   GAL-GFP-kd200-GALpA reporter
   

   Expression

   Yeast

   Mutation

   GFP is mutated such that amino acids 64-73 (FSYGV…

   Promoter

   bidirectional GAL1-GAL10 promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLD1 translation factors

   Plasmid

   #117760

   Purpose

   BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 1-13
   

   Use

   Escherichia coli

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmTurquoise2-N1

   Plasmid

   #60561

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTurquoise2-C1

   Plasmid

   #54842

   Purpose

   Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

   Depositor

   Michael Davidson, Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Type

   Empty backbone

   Tags

   mTurquoise2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBS-TRE-tdKatushka2-WPRE

   Plasmid

   #193337

   Purpose

   Expresses tdKatushka2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   tdKatushka2
   

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEVA221pmCDA1-T7RNAP

   Plasmid

   #167978

   Purpose

   Expresses fusion protein pmCDA1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   pmCDA1-T7RNAP (T7p07 Synthetic)
   

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmTurquoise2-NES

   Plasmid

   #36206

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   NES
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

   Plasmid

   #153526

   Purpose

   Expression of the anti SARS-CoV-2_RBD sybody Sb#45

   Depositor

   Markus Seeger

   Article

   Walter et al bioRxiv 2020.04.16.045419

   Insert

   Sb#45
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PKAcs-GGSD5X-GFP

    Plasmid

    #108584

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p12x601

    Plasmid

    #157785

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    pMD2.G(spei/spei)&12x601_tetO
    

    Expression

    Bacterial

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNAS1B

    Plasmid

    #61968

    Purpose

    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    mCherry
    GFP
    

    Expression

    Bacterial

    Mutation

    Serine 65 to Glycine (GGT), Serine 72 to Alanine …

    Promoter

    PBAD and PLtetO

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNVLTv2-pvdIJD-Prha-base

    Plasmid

    #169244

    Purpose

    Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    rhaRS
    

    Expression

    Bacterial

    Promoter

    rhaS

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEVA221rAPOBEC1-T7RNAP

    Plasmid

    #167979

    Purpose

    Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    rAPOBEC1-T7RNAP (T7p07 Synthetic)
    

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSK221

    Plasmid

    #129249

    Purpose

    reverse yTRAP, tetR-responsive Neon reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    pADH1(tetO2)-mNeonGreen
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAF259

    Plasmid

    #105494

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    Bitlucopt
    

    Use

    Atc-dependent expression in c. difficile

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-PDZD8HA_sh_resistant

    Plasmid

    #105006

    Purpose

    Mammalian Expression of PDZD8, sh resistant

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    PDZD8 (Pdzd8 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The sequence recognized by shPDZD8 (TRCN000043105…

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKL004

    Plasmid

    #98920

    Purpose

    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    GCaMP6f tethered to mRuby
    

    Tags

    His and mRuby3

    Expression

    Bacterial

    Promoter

    BBa_J23118 - Strong Constitutive

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

    Plasmid

    #153527

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#68

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#68
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge