We narrowed to 22,830 results for: Sis

1. pEBB XIAP W310A

   Plasmid

   #25677

   Depositor

   Colin Duckett

   Article

   Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Mutation

   W310A

   Available Since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pEBB XIAP W310A/H467A

   Plasmid

   #25682

   Depositor

   Colin Duckett

   Article

   Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Mutation

   W310A H467A

   Available Since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pGL2 enhancer- F6 Sp-1 mut

   Plasmid

   #22439

   Depositor

   William Sessa

   Article

   Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

   Insert

   F6 Sp-1 mut (NOS3 Human)
   

   Tags

   luciferase reporter gene

   Expression

   Mammalian

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5/FRT/TO SSA1

   Plasmid

   #19462

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   SSA1 (SSA1 Budding Yeast)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. xfz8 probe/pBS

   Plasmid

   #16323

   Depositor

   Peter Klein

   Article

   Deardorff et al Development. 1998 Jul . 125(14):2687-700

   Insert

   frizzled-8 (fzd8.S Frog)
   

   Expression

   Bacterial

   Mutation

   Base pairs 1159-3240 of xfz8 cDNA

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pET-21a(+) SurE D8A

   Plasmid

   #12685

   Depositor

   SeWon Suh

   Article

   Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

   Insert

   SurE (TM1662 )
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Aspartate 8 to Alanine

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pESdelta 799

   Plasmid

   #13042

   Depositor

   Peggy Hsieh

   Article

   Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

   Insert

   MutS
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C-terminal deletion from aa 800 through 853.

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pV1338

   Plasmid

   #111443

   Purpose

   Solo vector pV1326 + sgScADE2

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2
   

   Use

   CRISPR

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX Syt1 C2AB (96-421) F234C

   Plasmid

   #170644

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX Syt1 C2AB (96-421) V304C

    Plasmid

    #170645

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Syt1 C2AB (96-421) D303G F234C

    Plasmid

    #170647

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D303G, C277A, F234C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Syt1 C2AB (96-421) D303G V304C

    Plasmid

    #170648

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D303G, C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX Syt1 C2AB (96-421) D365E F234C

    Plasmid

    #170650

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D365E, C277A, F234C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Syt1 C2AB (96-421) D365E V304C

    Plasmid

    #170651

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D365E, C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX Syt1 C2AB (96-421) I367T F234C

    Plasmid

    #170653

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    I367T, C277A, F234C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX Syt1 C2AB (96-421) I367T V304C

    Plasmid

    #170654

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    I367T, C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C077

    Plasmid

    #174346

    Purpose

    Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (pabpc1.S Frog)
    

    Use

    In vitro transcription template

    Tags

    FLAG

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C078

    Plasmid

    #174347

    Purpose

    Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    ePAB
    

    Use

    In vitro transcription template

    Tags

    FLAG

    Mutation

    13 silent mutations: G243A, A273G, G339A, C345G, …

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C233

    Plasmid

    #174349

    Purpose

    Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (M161A) (pabpc1.S Frog)
    

    Use

    In vitro transcription template

    Tags

    FLAG

    Mutation

    M161A

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-TetOne-Puro-GFP

    Plasmid

    #171123

    Purpose

    Doxycycline inducible expression of GFP

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 2Flag-pDEST-C

    Plasmid

    #118372

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 2Flag-pDEST-N

    Plasmid

    #118371

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5-HA-UFM1

    Plasmid

    #134639

    Purpose

    expression of HA-tagged human UFM1 wildtype

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 (UFM1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRPML1-HA

    Plasmid

    #18825

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NG-ABE8e

    Plasmid

    #138491

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (NG)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-AAV9cap

    Plasmid

    #198016

    Purpose

    For high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM library

    Depositor

    George Church

    Article

    Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

    Insert

    AAV9 cap
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pBSHSP:Cre;CMV:tdTomato-SceI

    Plasmid

    #30525

    Depositor

    Gerhart Ryffel

    Article

    Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

    Insert

    Cre recombinase tdTomato
    

    Use

    Cre/Lox; Heat shock inducible, i-scei megabase ve…

    Expression

    Mammalian

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCW empty vector

    Plasmid

    #184708

    Purpose

    Lentiviral empty vector for doxycycline-inducible expression

    Depositor

    Alessia Ciarrocchi, Gloria Manzotti

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pQCXIP-GFP1-10

    Plasmid

    #68715

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    GFP1-10
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLminP_Luc2P_RE57

    Plasmid

    #90402

    Purpose

    Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    ISRE

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ABE8e

    Plasmid

    #138489

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEGFP-RhoA-C1

    Plasmid

    #29674

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

    Insert

    RhoA (RHOA Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid

    #205151

    Purpose

    Express UbC-driven LARGE1 and EF-1a driven BSD

    Depositor

    Monkol Lek

    Insert

    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFYF1320 EGFP Site#1

    Plasmid

    #47511

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEM705 Gsi (Ser)

    Plasmid

    #109352

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cys at position 391 to Serine (Pertussis …

    Promoter

    CAG

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-NLS-HA-Bxb1

    Plasmid

    #51271

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Bxb1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge