We narrowed to 43,221 results for: cha;

1. Opto-ACTU−

   Plasmid

   #198869

   Purpose

   Optogenetic recruitment construct to increase inner membrane surface charge

   Depositor

   Peter Devreotes

   Article

   Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

   Insert

   Rad17 polyanionic tail (-14)
   

   Use

   Synthetic Biology

   Tags

   CRY2PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMpGWB400

   Plasmid

   #68665

   Purpose

   MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMpGWB200

   Plasmid

   #68591

   Purpose

   MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQL47 EGFP-VAMP-GST

   Plasmid

   #122971

   Purpose

   Reporter of botulinum toxin B cleavage

   Depositor

   Chandra Tucker

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   EGFP-VAMP3-GST
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS-FLAG-CaMKIID

   Plasmid

   #186827

   Purpose

   Expresses FLAG-CaMKIID in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2d (Camk2d Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDAI-SceI-pheS

   Plasmid

   #111611

   Purpose

   Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Insert

   pheS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE-Stat5a1*6

   Plasmid

   #130668

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-FLEX-rev-ChR2-tdtomato

    Plasmid

    #18917

    Purpose

    Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-tdtomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    tdtomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB700 lenti gRNA Blasto

    Plasmid

    #167904

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

    Plasmid

    #168173

    Purpose

    Expression of BeCyclOp in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

    Insert

    BeCyclOp (codon optimized for C. elegans)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMpGWB109

    Plasmid

    #68563

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NAX_CAGGS_CAS9_Blasto

    Plasmid

    #167856

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. scFv-GCN4-sfGFP-TET1CD

    Plasmid

    #184439

    Purpose

    TET1 catalytic domain fused with sfGFP and scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    scFv-GCN4-sfGFP-TET1CD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMpGWB236

    Plasmid

    #68627

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEC2861 (pSpy1C-PgyrA_ffluc)

    Plasmid

    #218511

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PgyrA

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS-FLAG-CaMKIIB

    Plasmid

    #186826

    Purpose

    Expresses FLAG-CaMKIIB in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2b (Camk2b Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS178

    Plasmid

    #215678

    Purpose

    Empty repair plasmid for ChrII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxP + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p1D4-hrGFP II

    Plasmid

    #71317

    Purpose

    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.

    Depositor

    Belinda Chang

    Article

    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    1D4 epitope (TETSQVAPA) and IRES-hrGFPII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pEGFP/GCP60

    Plasmid

    #180633

    Purpose

    Expression of GCP60

    Depositor

    Carolyn Machamer

    Article

    Sbodio et al J Biol Chem. 2006 Sep 22;281(38):27924-3

    Insert

    GCP60 (ACBD3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-FLEX-rev-ChR2(H134R)-mCherry

    Plasmid

    #18916

    Purpose

    Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    mCherry

    Expression

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMpGWB104

    Plasmid

    #68558

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+) Drp1 V2

    Plasmid

    #44599

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 variant 2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Dronpa-Lifeact-7

    Plasmid

    #54740

    Purpose

    Localization: Actin, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

    Type

    Empty backbone

    Use

    Tags

    Dronpa-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-Vinculin-N-21

    Plasmid

    #55160

    Purpose

    Localization: Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pM355

    Plasmid

    #173175

    Purpose

    (Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    gRNA-12xMBS backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAIP-hXRCC1wt-eGFP

    Plasmid

    #206032

    Purpose

    Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMS59

    Plasmid

    #215680

    Purpose

    Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

    Plasmid

    #194247

    Purpose

    Expresses 3 miRNAs targeting human alpha-Synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRaSynuclein(human) (SNCA Human)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-IG-mSA

    Plasmid

    #80706

    Purpose

    Expresses monomeric streptavidin (mSA) in bacterial cells

    Depositor

    Matthieu Sainlos

    Article

    Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

    Insert

    Monomeric streptavidin
    

    Use

    Tags

    10xHis tag and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GST-OPTN

    Plasmid

    #171424

    Purpose

    For the purifcation of OPTN protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    OPTN (OPTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PT7-deGFP-MGapt

    Plasmid

    #67741

    Purpose

    T7 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T7term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pROSA26-CreERT2

    Plasmid

    #149437

    Purpose

    ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge