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pBad33_ISCvi2_reRNA

Plasmid

#205337

Purpose

Bacterial expression of ISCvi2 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISCvi2 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1

Plasmid

#203830

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E1

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E4

Plasmid

#203833

Purpose

Luciferase reporter plasmid for Igf1 enhancer 4

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E4

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E2

Plasmid

#203831

Purpose

Luciferase reporter plasmid for Igf1 enhancer 2

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E3

Plasmid

#203832

Purpose

Luciferase reporter plasmid for Igf1 enhancer 3

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E3

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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BPK2014-Cas12i2

Plasmid

#188275

Purpose

prokaryotic expression

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CcuCas9

Plasmid

#192127

Purpose

Expresses CcuCas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

CcuCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp2Cas9

Plasmid

#192126

Purpose

Expresses Hsp2Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1Cas9

Plasmid

#192125

Purpose

Expresses Hsp1Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSLS.601

Plasmid

#184955

Purpose

Express -Eco1 ncRNA library variants

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 ncRNA (variants)

Expression

Bacterial

Mutation

Library members with mutations described in manus…

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-sfGFP-SKL-Target2 (Mpphot)

Plasmid

#186722

Purpose

Transient expression vector for SKLPT imaging for target 2 (Mpphot) in plant cells (Negative control vector).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sfGFP-SKL-Mphot

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-sfGFP-SKL-Target1 (Mpphot)

Plasmid

#186721

Purpose

Transient expression vector for SKLPT imaging for target 1 (Mpphot) in plant cells (Positive control vector).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sfGFP-SKL-Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target2 (Mpphot)

Plasmid

#186727

Purpose

Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target1 (Mpphot)

Plasmid

#186726

Purpose

Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/Zeo-sfGFP-SKL

Plasmid

#186720

Purpose

Gateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sfGFP-SKL

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS538

Plasmid

#188881

Purpose

Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Cas9

Use

Gateway entry vector

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.22

Plasmid

#183566

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 22

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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