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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pQmod2S-GG

Plasmid

#191346

Purpose

Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

Plasmid

#162473

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreAB

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

Plasmid

#162478

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreAB

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw

Plasmid

#162476

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreB

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

Plasmid

#162475

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreA

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

Plasmid

#162472

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreC

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

Plasmid

#162470

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreA

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

Plasmid

#162477

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreC

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

Plasmid

#162471

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreB

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00223

Plasmid

#34371

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG30443 (Opbp Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-p14

Plasmid

#42330

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p14 (LAMTOR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-mp1

Plasmid

#42329

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

mp1 (LAMTOR3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pYZ145

Plasmid

#98405

Purpose

Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Inserts

upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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VanGlow-GL

Plasmid

#83342

Purpose

for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

Depositor

Cheng-Yu Lee

Article

Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

Type

Empty backbone

Use

Luciferase

Tags

GFP::Luciferase(nls)

Expression

Insect

Mutation

Promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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VanGlow-RR

Plasmid

#83343

Purpose

for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

Depositor

Cheng-Yu Lee

Article

Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

Type

Empty backbone

Use

Luciferase

Tags

mCherry::Renilla(nls)

Expression

Insect

Mutation

Promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a-mCherry-MED1-IDR

Plasmid

#194545

Purpose

Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MED1 (MED1 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_KLF6

Plasmid

#167772

Purpose

Expresses KLF6 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

KLF6 (KLF6 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPBbleo-ErbB2

Plasmid

#197361

Purpose

Encoding human ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 MP1 shRNA

Plasmid

#26632

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

MP1 shRNA (LAMTOR3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only