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Showing: 401 - 420 of 6924 results

  1. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

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  2. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid
    #233035
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233035-plasmid-map-sequence-id-467423

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  5. pAAV-EFS-DjCas13d-P2A-mCherry-pA

    Plasmid
    #233027
    Purpose
    To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d/mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233027-plasmid-map-sequence-id-463789

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  6. pAAV-EFS-CasRx-P2A-mCherry-pA

    Plasmid
    #233025
    Purpose
    To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site
    Depositor
    Jonathan Ploski
    Insert
    CasRx/mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233025-plasmid-map-sequence-id-463770

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  7. pAAV-EFS-hfCas13d-P2A-mCherry-pA

    Plasmid
    #233026
    Purpose
    To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d/mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233026-plasmid-map-sequence-id-463769

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  8. pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233032
    Purpose
    To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    CasRx and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233032-plasmid-map-sequence-id-463767

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  9. FFTTH-Amotl2

    Plasmid
    #112832
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112832-plasmid-map-sequence-id-217935

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  10. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  12. FFTTH--GFP-Amotl2

    Plasmid
    #112833
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112833-plasmid-map-sequence-id-217927

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  13. TAP-GFP-Amotl2

    Plasmid
    #112835
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    GFP and TAP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112835-plasmid-map-sequence-id-217938

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  14. pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233033
    Purpose
    To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233033-plasmid-map-sequence-id-464626

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  15. pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233034
    Purpose
    To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233034-plasmid-map-sequence-id-463768

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  16. TAP-Amotl2

    Plasmid
    #112834
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    TAP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112834-plasmid-map-sequence-id-217933

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  17. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid
    #233039
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233039-plasmid-map-sequence-id-463773

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  18. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  19. pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

    Plasmid
    #233038
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233038-plasmid-map-sequence-id-463774

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  20. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid
    #183704
    Purpose
    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM4D(Gi)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183704-plasmid-map-sequence-id-360830

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Showing: 401 - 420 of 6924 results