We narrowed to 10,828 results for: 158

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA509

Plasmid

#231116

Purpose

Fragmid fragment: (2A Selection) ABE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTrc-clbS_Frischella

Plasmid

#223358

Purpose

Encoding ClbS from Frischella perrara

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

Encoding ClbS from Frischella perrara (Codon optimized for E coli)

Expression

Bacterial

Promoter

pTrc

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-clbS_Mixta

Plasmid

#223352

Purpose

Encoding ClbS from Mixta theicola

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

Encoding ClbS from Mixta theicola (Codon optimized for E coli)

Expression

Bacterial

Promoter

pTrc

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-clbS

Plasmid

#223350

Purpose

Encoding wild type ClbS from E. coli CFT073

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

ClbS from CFT073 (Codon optimized for E coli)

Expression

Bacterial

Promoter

pTrc

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKDC.118

Plasmid

#227188

Purpose

T7 IPTG-driven Cas13a

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

LbuCas13a

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB170

Plasmid

#218591

Purpose

Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Adr1

Expression

Yeast

Mutation

Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1340

Plasmid

#218593

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa50H9

Plasmid

#179628

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa50H9

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa3G5

Plasmid

#179629

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa3G5

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa30F3

Plasmid

#179620

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa30F3

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa32G3

Plasmid

#179621

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa32G3

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAb132A11

Plasmid

#179622

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAb132A11

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa45C8S

Plasmid

#179623

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa45C8S

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HULAa2F4

Plasmid

#179624

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa2F4

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HULAa36F11

Plasmid

#179625

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa36F11

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EINA20F1

Plasmid

#179626

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

EINA20F1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EINA62G7

Plasmid

#179627

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

EINA62G7

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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SNAPf-BASU-His

Plasmid

#218450

Purpose

E coli expression vector for SNAPf-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAPf-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUCIDT-AMP-Fp16SrRNA

Plasmid

#218519

Purpose

pUCIDT-AMP containing 16S rRNA gene of F. prausnitzii for absolute concentration quantification

Depositor

Ami Bhatt

Article

Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

Insert

16S rRNA

Use

Synthetic Biology

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSK-Spsg

Plasmid

#214351

Purpose

Expresses cloning backbone for SpCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9 tracrRNA

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge