We narrowed to 165,688 results for: Gene

1. lentiCRISPR-v2-HygR-mCherry

   Plasmid

   #167189

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDRM166 LKB (Luciferase mKate2 Blast)

   Plasmid

   #183502

   Purpose

   Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   mKate2
   BSD
   

   Use

   Lentiviral and Luciferase

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC-G418-YR

   Plasmid

   #61767

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   nptII gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Promoter

   trpC promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5xQUAS-ZsGreen-P2A, α-cry:mCherry

   Plasmid

   #180477

   Purpose

   Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Inserts

   5x QUAS-ZsGreen-P2A
   mCherry
   

   Use

   Synthetic Biology

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

   Plasmid

   #111818

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Mef2c (Mef2c Mouse)
   Tdtomato
   Gata4 (Gata4 Mouse)
   Tbx5
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHEE401

   Plasmid

   #71286

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p13xQUAS-ZsGreen-P2A, α-cry:mCherry

   Plasmid

   #180480

   Purpose

   Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   13x QUAS-ZsGreen-P2A
   

   Use

   Synthetic Biology

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p13xQUAS-MCS, α-cry:mCherry

   Plasmid

   #180481

   Purpose

   Inducible gene expression vector p13xQUAS-MCS, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   Multiple cloning site
   

   Use

   Synthetic Biology

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

   Plasmid

   #218793

   Purpose

   AAV reporter genome plasmid

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   NLSf-mScarletP2A-hLuc
   

   Use

   AAV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQFDBD-2x AD*-VP16*, α-cry:EGFP

    Plasmid

    #180473

    Purpose

    Gene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    QFDBD-2xQFAD*-VP16*
    

    Use

    Synthetic Biology

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p9xQUAS-ZsGreen-P2A, α-cry:mCherry

    Plasmid

    #180479

    Purpose

    Inducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    9x QUAS-ZsGreen-P2A
    

    Use

    Synthetic Biology

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p17xQUAS-Luciferase, α-cry:mCherry

    Plasmid

    #180482

    Purpose

    Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Inserts

    Luciferase
    17x QUAS
    

    Use

    Synthetic Biology

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid

    #64222

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-TAZ-CAMTA1

    Plasmid

    #235680

    Purpose

    Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

    Depositor

    Duojia Pan

    Article

    Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

    Insert

    TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCA.66/2272

    Plasmid

    #22733

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox66-pU(delta)TK-EM7Neo-Lox2272
    

    Use

    Cre/Lox; Bac recombineering

    Mutation

    This vector was designed for assembling gene targ…

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid

    #102851

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

    Plasmid

    #64220

    Purpose

    Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64219

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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