We narrowed to 6,061 results for: WUS;

1. pAAV-hSyn-DIO-GRAB_ACh3.0

   Plasmid

   #121923

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-CBA-hGBA1-HA-hGHpA

   Plasmid

   #218794

   Purpose

   AAV reporter genome plasmid

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   CMV-CBA-hGBA1-HA-hGHpA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. eTd-CBE

   Plasmid

   #196601

   Purpose

   Mammalian expression of eTd-CBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSW031

   Plasmid

   #231144

   Purpose

   Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

   Depositor

   Philip Cole

   Article

   Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

   Insert

   S. aureus Sortase A mutant
   

   Use

   Tags

   Nostoc punctiform split intein C-terminal fragmen…

   Expression

   Bacterial

   Mutation

   P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

   Promoter

   T7

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-haA3A-CBE-G-C

   Plasmid

   #220899

   Purpose

   AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-G-C
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. gGBEv6.3-SpG

   Plasmid

   #202630

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpG(D10A)-MPGv6.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

   Promoter

   hU6, EF1a, CBh

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-117G-hyeA3A-BE4max

   Plasmid

   #157946

   Purpose

   Lentiviral expression of hyeA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   Lenti -117G-hyeA3A-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDYT001

   Plasmid

   #186549

   Purpose

   Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)

   Depositor

   Jonathan Weissman

   Article

   Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

   Insert

   14-bp random integration barcode and three target sites and 3x sgRNAs
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

    Plasmid

    #228254

    Purpose

    pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACBE

    Plasmid

    #204607

    Purpose

    Mammalian expression of adenine-to-cytosine base editor ACBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. A&C-BEmax

    Plasmid

    #157947

    Purpose

    Mammalian expression of A&C-BEmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A&C-BEmax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Td-CGBE

    Plasmid

    #196599

    Purpose

    Mammalian expression of Td-CGBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L); S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eTd-CBEm

    Plasmid

    #196602

    Purpose

    Mammalian expression of eTd-CBEm

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti6/V5-DEST-HSF1

    Plasmid

    #31210

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

16. eTd-CBEa

    Plasmid

    #196603

    Purpose

    Mammalian expression of eTd-CBEa

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-PD-1 (Y223F, Y248F)-mGFP

    Plasmid

    #180790

    Purpose

    Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1 (Y223F, Y248F) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIG-641 JE-TRAC-BCMA(BB)28z-P2A

    Plasmid

    #186127

    Purpose

    BCMA-CAR DNA production

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    BCMA-CAR (TNFRSF17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-FAB(PX-LD)

    Plasmid

    #214422

    Purpose

    Detection of peroxisome-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and FKBP-V5-RspAN

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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