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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,759 results for: nls

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Showing: 8401 - 8440 of 18759 results

  2. L4796

    Plasmid
    #1680
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    let858enh>>, let858enh<<, let858-455,…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1680-plasmid-map-sequence-id-290

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  3. L2603

    Plasmid
    #1511
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, ATG-SecSig, pes10
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1511-plasmid-map-sequence-id-121

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  4. L3135

    Plasmid
    #1531
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, ATG-SecSig, pes10
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1531-plasmid-map-sequence-id-141

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  5. CAG-eeBxb1_bGH

    Plasmid
    #252238
    Purpose
    eeBxb1-expressing plasmid under CAG promoter
    Depositor
    Kate Galloway
    Insert
    eeBxb1
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    July 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    252238-plasmid-map-sequence-id-504775

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  15. snuABE4.2

    Plasmid
    #246014
    Purpose
    snuABE4.1 with MLH1-SB fused to the C-terminus via P2A.
    Depositor
    Sangsu Bae
    Publication
    Im et al bioRxiv 2025.07.19.664718
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Tags
    MLH1-SB
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246014-plasmid-map-sequence-id-492308

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  20. pSLQ14256

    Plasmid
    #239275
    Purpose
    Expresses Kif5a*-PYL1 for the anterograde transport of RNA along microtubules via CRISPR-TO in primary mouse cortical neurons
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    Kif5a(aa1-559)-BFP-PYL1 (Kif5a Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239275-plasmid-map-sequence-id-474751

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  23. Td-CBE-NW

    Plasmid
    #240623
    Purpose
    Cytidine base editor with narrowed editing window
    Depositor
    Tingting Jiang
    Publication
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-8e (E27R, N46L, N108Q, M151C, Q154Y)-linker-nCas9-P2A-UGI
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA-8e (E27R, N46L, N108Q, M151C, Q154Y), Cas9-P…
    Available since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    240623-plasmid-map-sequence-id-480852

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  24. pHS1993

    Plasmid
    #239836
    Purpose
    OMEGAOff-KRK mammalian expression
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    Tags
    HA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239836-plasmid-map-sequence-id-479108

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  26. pHR_SFFV_tTA_IRES_tagBFP

    Plasmid
    #231953
    Purpose
    Lentiviral vector for constitutive expression of the tetracycline transactivator tTA and of the blue fluorescent protein tagBFP, separated by an internal ribosome entry site (SFFV promoter)
    Depositor
    Leonardo Morsut
    Insert
    tTA_IRES_tagBFP
    Use
    Lentiviral
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231953-plasmid-map-sequence-id-470256

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  29. pYPQAT51

    Plasmid
    #223423
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223423-plasmid-map-sequence-id-453500

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  30. pYPQAT31

    Plasmid
    #223403
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223403-plasmid-map-sequence-id-454164

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  38. evoBE4-NG

    Plasmid
    #226852
    Purpose
    Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker
    Depositor
    Alexis Komor
    Publication
    Osgood et al Methods Enzymol. 2025;712:351-404. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    evoBE4max-NG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226852-plasmid-map-sequence-id-450496

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  40. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Publication
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    225958-plasmid-map-sequence-id-452543

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Showing: 8401 - 8440 of 18759 results