We narrowed to 4,397 results for: GCA;

Showing: 4201 - 4220 of 4397 results

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pORANGE GFP-CACNA1E KI

Plasmid

#131481

Purpose

Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

Plasmid

#163179

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-1

Plasmid

#223223

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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sgTP53(B)

Plasmid

#85560

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pRSITEP-puro-shWRN1

Plasmid

#125783

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-3

Plasmid

#118021

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-3 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-TGFBR3

Plasmid

#23478

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TGFBR3 (TGFBR3 Human)

Use

Gateway donor vector

Mutation

C44T, 1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRSITEP-puro-shWRN1-C911

Plasmid

#125784

Purpose

a seed-matched control for pRSITEP-puro-shWRN1

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1-C911 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST Halo-flag-CAD

Plasmid

#188117

Purpose

Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag and Halo tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-5

Plasmid

#118023

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-5 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-rMERTK-HITI

Plasmid

#87119

Purpose

Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA

Use

AAV and CRISPR

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN1

Plasmid

#125781

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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sgEGFR(A)

Plasmid

#85564

Purpose

Expresses sgRNA targeting human EGFR around codon T790

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

epidermal growth factor receptor (EGFR Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

Plasmid

#211986

Purpose

Express gRNA against SOX2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting SOX2 (SOX2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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sgCRY1

Plasmid

#176511

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY1-2 (Cry1, Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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FGFR1 gRNA (BRDN0001145494)

Plasmid

#76067

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR1 (FGFR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001147841)

Plasmid

#76220

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer20_SLC38A2_wt

Plasmid

#156182

Purpose

Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

TRE

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD E1368A

Plasmid

#188141

Purpose

Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AURKB gRNA (BRDN0001146837)

Plasmid

#77619

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKB (AURKB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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