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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,953 results for: NOG;

Showing: 4221 - 4240 of 4953 results

  1. pCAT079

    Plasmid
    #180509
    Purpose
    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180509-plasmid-map-sequence-id-364076

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  3. pMT-FLAG-CBPΔ(N+HAT)

    Plasmid
    #182841
    Purpose
    An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    CBP (w Fly)
    Use
    Tags
    FLAG tag
    Expression
    Insect
    Mutation
    dletion of residues 1566-2142 from CBP1476-3222
    Promoter
    MT
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182841-plasmid-map-sequence-id-360051

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  4. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

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  19. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid
    #136884
    Purpose
    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)
    Depositor
    Moritz Mall
    Article
    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi
    Insert
    engrailed repressor domain (en Fly)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    136884-plasmid-map-sequence-id-272359

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Showing: 4221 - 4240 of 4953 results