We narrowed to 4,338 results for: Pet2

1. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. proISG15 (2-165)

   Plasmid

   #110762

   Purpose

   Bacterial expression of proISG15

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Tags

   N-His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6tev CBP BCHZ (1079-1751)

   Plasmid

   #99341

   Purpose

   Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
   

   Tags

   His6tev

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Met1 diUb (1-152)

   Plasmid

   #110766

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Tags

   N-His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. H6SUMO-IntC-P53(304-393)

   Plasmid

   #200316

   Purpose

   bacterial expression of P53 TET-CTD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (304-393) (TP53 Human)
   

   Tags

   His6-SUMO and designed intein C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. H6GB1tevP53(1-303)IntN

   Plasmid

   #200312

   Purpose

   Bacterial expression of P53 NTAD-DBD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (TP53 Human)
   

   Tags

   His6GB1tev and designed intein N

   Expression

   Bacterial

   Mutation

   M133L, V203A, N239Y, N268D

   Promoter

   T7

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJG1 T7-8xHis-OGT D554N

   Plasmid

   #154279

   Purpose

   Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   Aspartate 554 changed to Asparagine- bacterial co…

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3I91

   Plasmid

   #62514

   Purpose

   Bacterial expression plasmid for use in structural determination.

   Depositor

   Cheryl Arrowsmith

   Article

   Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

   Insert

   CBX8 (CBX8 Human)
   

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. His6tev CBP BCHZDeltaAL

    Plasmid

    #99551

    Purpose

    Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
    

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    replaced 1557-1618 with SGGSG

    Promoter

    T7

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN672

    Plasmid

    #177362

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper and His tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Tags

    His tag and Leucine zipper

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-GINA3

    Plasmid

    #74980

    Purpose

    Expresses His tagged GINA3 protein in bacteria.

    Depositor

    Pradipta Ghosh

    Article

    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

    Insert

    Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. His6-SUMO-SPMc-SpyCatcher003-Ctag

    Plasmid

    #246648

    Purpose

    Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation

    Depositor

    Mark Howarth

    Article

    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

    Insert

    His6-SUMO-SPMc-SpyCatcher003-Ctag
    

    Tags

    Ctag, His-tag, SUMO, and SpyCatcher003

    Expression

    Bacterial

    Mutation

    The SPMc comprises the C-terminal fragment of th…

    Promoter

    T7

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. TTR C10S S85C F87E

    Plasmid

    #238074

    Purpose

    Bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S S85C F87E

    Promoter

    T7

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ce iPGM-C-HiBiT

    Plasmid

    #207120

    Purpose

    Bacterially expressed iPGM-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    

    Tags

    HiBiT Tag and Thrombin cleavage site + 10x His Ta…

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. E. coli Lig-N-HiBiT

    Plasmid

    #215437

    Purpose

    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    

    Tags

    10xHis-tag with thrombin site and HiBiT Tag

    Expression

    Bacterial

    Mutation

    Codon Optimized for bacterial expression, T7 gene…

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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