We narrowed to 114,331 results for: MPI

1. pAAV-CMV-rtTA-IRES-GFP-WPRE-pA

   Plasmid

   #183709

   Purpose

   AAV designed to express rtTA and eGFP from a CMV promoter

   Depositor

   Jonathan Ploski

   Insert

   rtTA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   P45
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5/FRT/TO-neo (1795)

   Plasmid

   #41000

   Depositor

   Jonathon Pines

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-fDIO mCherry

   Plasmid

   #114471

   Purpose

   Flp-dependent mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PH-PLC-delta-EGFP

   Plasmid

   #136998

   Purpose

   Lentiviral EGFP lipid sensor for PI(4,5)P2

   Depositor

   Ken-Ichi Takemaru

   Insert

   PH-PLC-delta-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBOH-LIC

   Plasmid

   #26099

   Purpose

   SGC Empty baculovirus expression/transfer vector

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   6x His and Thrombin protease cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pFA6a-mNeonGreen-KanMX6

   Plasmid

   #129099

   Purpose

   For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

   Depositor

   Wei-Lih Lee

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22B_SA_PcrA

   Plasmid

   #102999

   Purpose

   The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

   Depositor

   Martin Webb

   Insert

   SA_PcrA
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   PcrA 199-2423

   Promoter

   T7

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-APEX3-C Cloning Vector

   Plasmid

   #220615

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Flag-APEX3 fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3 HA-humanTRAF3 (1098)

    Plasmid

    #66927

    Purpose

    Mammalian Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-spCas9-pNeo

    Plasmid

    #214120

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid

    #101893

    Purpose

    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid

    #78113

    Purpose

    Nt His-tag Eptesicus fuscus Sacsin 89-1368

    Depositor

    Kalle Gehring

    Insert

    Sacsin Eptesicus fuscus 89-1368
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiGuide_puro-P2A-mNeonGreen

    Plasmid

    #221464

    Purpose

    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker

    Depositor

    James Eshleman

    Insert

    mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LT3GEPIR-NEK7shRNA1

    Plasmid

    #220550

    Purpose

    To inducibly knockdown NEK7 expression

    Depositor

    Sean Lee

    Insert

    NEK7 shRNA1 (NEK7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Peripherin/pBSSK+

    Plasmid

    #98132

    Purpose

    Peripherin (NM_006262) in pBSSK+

    Depositor

    Rita Shiang

    Insert

    Peripherin (PRPH Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid

    #196724

    Purpose

    Sortable and selectable EnAsCas12a.

    Depositor

    Bernhard Schmierer

    Insert

    enASCas12a-HA-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS - HA and SV40 NLS

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUGW-H2B-mCherry

    Plasmid

    #132333

    Purpose

    Third generation lentiviral vector for hUbC-driven H2B-mCherry expression

    Depositor

    Bjoern Schwer

    Insert

    H2B-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    July 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCVhyg-DEST

    Plasmid

    #119747

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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