We narrowed to 15,088 results for: pET

1. pKHH030_sgCtrl#2

   Plasmid

   #174143

   Purpose

   Lentiviral vector expressing a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiGuide-Neo-NCOA7g3 (BB36)

   Plasmid

   #139454

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide-Neo-NCOA7g2 (BB35)

   Plasmid

   #139453

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCUU

   Plasmid

   #159747

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/NDUFS2 _34-66/myc-His

   Plasmid

   #127495

   Purpose

   Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 _34-66 (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 _34-66

   Promoter

   CMV

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/NDUFS2 E57A/myc-His

   Plasmid

   #127497

   Purpose

   Expresses myc-tagged NDUFS2/E57A in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 E57A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 E57A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/NDUFS2 T58A/myc-His

   Plasmid

   #127498

   Purpose

   Expresses myc-tagged NDUFS2/T58A in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 T58A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 T58A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/NDUFS2 H60A/myc-His

   Plasmid

   #127499

   Purpose

   Expresses myc-tagged NDUFS2/H60A in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 H60A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 H60A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/NDUFS2 W61A/myc-His

   Plasmid

   #127500

   Purpose

   Expresses myc-tagged NDUFS2/W61A in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 W61A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 W61A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1/NDUFS2 K62A/myc-His

    Plasmid

    #127501

    Purpose

    Expresses myc-tagged NDUFS2/K62A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 K62A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 K62A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/NDUFS2 P63A/myc-His

    Plasmid

    #127502

    Purpose

    Expresses myc-tagged NDUFS2/P63A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 P63A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 P63A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

    Plasmid

    #117324

    Purpose

    CRISPR-Cas9 for miR-124-1, 3'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1-3' (MIR124-1 Synthetic)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

    Plasmid

    #117322

    Purpose

    CRISPR-Cas9 for miR-124-1/2, 5'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP Kind2 S159/181/666E

    Plasmid

    #105314

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666E

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti CGIP + Z-AAT target

    Plasmid

    #86007

    Purpose

    lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    Z-AAT target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti CGIP + TTR target

    Plasmid

    #86009

    Purpose

    lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    TTR target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NC11 pCDNA3.1 VENUS WTX K292N

    Plasmid

    #36966

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX K292N (AMER1 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    K292N

    Promoter

    CMV

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. NC8 pCDNA3.1 VENUS WTX S286D

    Plasmid

    #36963

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX S286D (AMER1 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    S286D

    Promoter

    CMV

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. NC7 pCDNA3.1 VENUS WTX S286A

    Plasmid

    #36962

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX S286A (AMER1 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    S286A

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

21. NC10 pCDNA3.1 VENUS WTX T289A

    Plasmid

    #36965

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX T289A (AMER1 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T289A

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

22. NC9 pCDNA3.1 VENUS WTX S286E

    Plasmid

    #36964

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX S286E (AMER1 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    S286E

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

23. 6xHis-MBP-BrCas12b-FNLDTA

    Plasmid

    #195340

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis Tag and MBP, TEV cleavage site, 6x His Tag

    Expression

    Bacterial

    Mutation

    F208W, N524V, L795I, D868V, T874S, A1015E

    Promoter

    T7 Promoter

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHis6-SUMO-g0s2

    Plasmid

    #179293

    Purpose

    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

    Insert

    G0S2 (G0S2 Human)
    

    Tags

    His6 + SUMO + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    full-length G0S2, residues 1-103 from accession n…

    Promoter

    T7

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEMS1132

    Plasmid

    #29053

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CHFR-PBZ-eGFP

    Plasmid

    #211581

    Purpose

    Expressing CHFR PBZ domain fused to eGFP

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    CHFR PBZ domain (CHFR Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    PBZ domain of the protein

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Shh

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pINTO-N3::hTET3

    Plasmid

    #136364

    Purpose

    Expresses epitope-tagged human TET3 WT in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-sgKeap1#1/Cre

    Plasmid

    #173639

    Purpose

    Expresses a Keap1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Keap1 (Keap1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

    Plasmid

    #204821

    Purpose

    Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

    Depositor

    Emma Rawlins

    Article

    He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

    Insert

    An upstream region including human SCGB3A2 promoter
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SCGB3A2 promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Lenti-sgBrca2#1/Cre

    Plasmid

    #173627

    Purpose

    Expresses a Brca2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Brca2 (Brca2 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

    Plasmid

    #236246

    Purpose

    Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    U6 and human Synapsin1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lenti-sgBrca2#2/Cre

    Plasmid

    #173628

    Purpose

    Expresses a Brca2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Brca2 (Brca2 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSMALB-ATF4.12

    Plasmid

    #155033

    Purpose

    Fluorescent reporter for ATF4 translation, Negative Control

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    uORF1 start codon mutated

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSMALB-ATF4.14

    Plasmid

    #155034

    Purpose

    Fluorescent reporter for ATF4 translation, Positive Control

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    uORF1 and uORF2 start codons mutated

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCVpuro-Mito-Pericam

    Plasmid

    #87381

    Purpose

    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    Mito-Pericam [ratiometric]
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Retroviral 5' LTR promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Lenti-sgCrebbp#2/Cre

    Plasmid

    #193210

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgCrebbp#2 (Crebbp Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Lenti-sgArid1a#1/Cre

    Plasmid

    #173571

    Purpose

    Expresses a Arid1a-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Arid1a (Arid1a Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204190

    Purpose

    Doxycycline inducible lentiviral vector for human TFAP2C overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid

    #210567

    Purpose

    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

    Plasmid

    #236231

    Purpose

    AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBiFC-PBZ-4A-VC

    Plasmid

    #110649

    Purpose

    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Tags

    HA and VC

    Expression

    Mammalian

    Mutation

    C379A, C385A, C421A and C427A

    Promoter

    CMV

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSMPUW-miR-124-GFP-Puro

    Plasmid

    #117321

    Purpose

    Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-124-1 (MIR124-1 Human)
    

    Use

    Lentiviral

    Tags

    GFP-Puro

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. Lenti-sgCrebbp#1/Cre

    Plasmid

    #193209

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgCrebbp#1 (Crebbp Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

    Plasmid

    #139444

    Purpose

    Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    sequence codon-optimized

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

    Plasmid

    #236232

    Purpose

    AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mScarletI

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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