We narrowed to 4,589 results for: LUM

1. iRFP-FRB-ER

   Plasmid

   #139317

   Purpose

   endoplasmic reticulum-resident FRB for chemically induced dimerization

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC32-Kar2-CFP-HDEL

   Plasmid

   #96989

   Purpose

   Express endoplasmic reticulum (ER) marker in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   Kar2-CFP-HDEL
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

   Plasmid

   #175530

   Purpose

   Expression of HaloTag7 in the endoplasmid reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag7-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIgnaviCas9

   Plasmid

   #127595

   Purpose

   Expresses IgnaviCas9 in E. coli

   Depositor

   Stephen Quake

   Article

   Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

   Insert

   IgnaviCas9
   

   Use

   Tags

   6xHis Tag and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LiRT

   Plasmid

   #214075

   Purpose

   Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LiRT_RTctrl

   Plasmid

   #214078

   Purpose

   Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LiRTi

   Plasmid

   #214076

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LiRTX_ctrl

   Plasmid

   #214077

   Purpose

   Luminescent reporter system, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRATIO3267

   Plasmid

   #141312

   Purpose

   Gateway cloning compatible dual bioluminescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Use

   Tags

   gLUC and redLUC

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQIq_MRGS_HIS8_(27F3)_FLAG

    Plasmid

    #199615

    Purpose

    Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27F3
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRATIO1267

    Plasmid

    #141301

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    gLUC and redLUC

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQIq_MRGS_HIS8_(27G2)_FLAG

    Plasmid

    #199616

    Purpose

    Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27G2
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-ER-rsTagRFP

    Plasmid

    #87620

    Purpose

    Endoplasmic reticulum (ER)-targeted rsTagRFP.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    ER-rsTagRFP
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAPV-DsRed2-FLAG-ER-5

    Plasmid

    #170622

    Purpose

    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PRLSS-SH2-KDEL

    Plasmid

    #209716

    Purpose

    Mammalian expression of SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQIq_MRGS_HIS8_( E3_5)_FLAG

    Plasmid

    #199612

    Purpose

    Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG E3_5 (control)
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL50016

    Plasmid

    #50315

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Codon-optimised for A. thaliana. BsaI/BbsI sites …

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-7aa-Cb5-pEGFP-C1

    Plasmid

    #166764

    Purpose

    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5a Rat)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mPapaya-CytERM-N-17

    Plasmid

    #56345

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    CytERM
    

    Use

    Tags

    mPapaya

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only