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pAc5.1B-lambdaN-HA-DmTral_394-652_D

Plasmid

#146139

Purpose

Insect Expression of DmTral_394-652

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-652 (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-R21EE23K_C

Plasmid

#146078

Purpose

Insect Expression of DmTral-R21EE23K

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R21EE23K (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-R21ES13AI15A_C

Plasmid

#146079

Purpose

Insect Expression of DmTral-R21ES13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R21ES13AI15A (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-R42EF44A_C

Plasmid

#146080

Purpose

Insect Expression of DmTral-R42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42EF44A (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-S13AI15A_C

Plasmid

#146081

Purpose

Insect Expression of DmTral-S13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-S13AI15A (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

Plasmid

#146071

Purpose

Insect Expression of DmTral-LSmR21EE23KR42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KR42EF44A (tral Fly)

Expression

Insect

Mutation

R21E, E23K, R42E, F44A. Additionally, one silent …

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R42EF44A_C

Plasmid

#146061

Purpose

Insect Expression of DmTral-R42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42EF44A (tral Fly)

Expression

Insect

Mutation

R42E, F44A. Additionally, two silent mutations A1…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

Plasmid

#146049

Purpose

Insect Expression of DmTral-LSmR21EE23KS13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KS13AI15A (tral Fly)

Expression

Insect

Mutation

S13A, I15A, R21E, E23K, one silent mutation A198C…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

Plasmid

#146039

Purpose

Insect Expression of DmTral-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDF-ADA (tral Fly)

Expression

Insect

Mutation

FDF to ADA (407-409) mutation. Additionally, thre…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-E383K

Plasmid

#113731

Purpose

Express mutant RGS7- E383K- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E383K

Promoter

CMV

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-R44C

Plasmid

#113729

Purpose

Express mutant RGS7- R44C- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-R310_RASA2-EF1-Puro

Plasmid

#114097

Purpose

Express mutant RASA2 in mamalian cells

Depositor

Yardena Samuels

Article

Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

Insert

RASA2 (RASA2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R310*

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

Plasmid

#112987

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

S123-125-3A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

Plasmid

#112985

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

S123-125-3D

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15D

Plasmid

#113010

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to D

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAc-GFP-dSUMOdeltaC

Plasmid

#85697

Purpose

expression of GFP-SUMO1 delta C in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Tags

GFP

Expression

Insect

Mutation

non-conjugatable SUMO, lacking the C-terminal gly…

Promoter

pAc5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
KHBD00320

Plasmid

#34445

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6376 (E2f1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only