We narrowed to 9,512 results for: try

1. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse

   Plasmid

   #238133

   Purpose

   This plasmid encodes the full-length Mouse (Mus musculus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B (Tmem106b Mouse)
   

   Use

   Lentiviral

   Tags

   Blasticidin-P2A-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

   Plasmid

   #202555

   Purpose

   Synaptotagmin-1 sgRNA2 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

   Plasmid

   #202556

   Purpose

   Synaptotagmin-1 sgRNA3 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

   Plasmid

   #202554

   Purpose

   Synaptotagmin-1 sgRNA1 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

   Plasmid

   #127343

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   None

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXBI-mclover-YTH-IDR2

   Plasmid

   #177122

   Purpose

   Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
   

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   YTH and IDR2 domains only (IDR1 deletion)

   Promoter

   T7

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFOSGE_COF2F04

   Plasmid

   #70901

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   mod(mdg4) (mod(mdg4) Fly)
   

   Use

   Gateway entry vector

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLV[TetOn]-Puro-TRE3G>mAscl1

   Plasmid

   #198755

   Purpose

   Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Promoter

   TRE3G

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV[TetOn]-Puro-TRE3G>mNeurog2

   Plasmid

   #198754

   Purpose

   Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Neurogenin-2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Promoter

   TRE3G

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4-eIF3B

    Plasmid

    #133308

    Purpose

    Expression of luciferase driven by eIF3B promoter region

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter (EIF3B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    eIF3B

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid

    #237480

    Purpose

    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    

    Use

    Lentiviral

    Tags

    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin

    Expression

    Mammalian

    Mutation

    S484D + S813I + deleted amino acids 68-76 + 676-6…

    Promoter

    CMV

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid

    #237479

    Purpose

    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Lentiviral

    Tags

    P2A-Blasticidin

    Expression

    Mammalian

    Mutation

    M210A + F213A

    Promoter

    CMV

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4-eIF3B MBE3 mutant

    Plasmid

    #133310

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE3 mutant (EIF3B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    ccacgtgacc changed to cAaAAAAacc

    Promoter

    eIF3B

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4-eIF3B MBE2 mutant

    Plasmid

    #133309

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE2 mutant (EIF3B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    gccacatgcacc changed to gcAaAaAAcacc

    Promoter

    eIF3B

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid

    #202553

    Purpose

    Nontargeting control vector with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Mutation

    Puromycin-resistance gene in the pLV hU6-sgRNA hU…

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOTag2T

    Plasmid

    #26295

    Depositor

    George Cross

    Type

    Empty backbone

    Use

    Trypanosome in-situ tagging vector

    Tags

    Ty-1

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOTag2H

    Plasmid

    #26296

    Depositor

    George Cross

    Type

    Empty backbone

    Use

    Trypanosome in-situ tagging vector

    Tags

    Triple HA

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOTag5H

    Plasmid

    #26299

    Depositor

    George Cross

    Type

    Empty backbone

    Use

    Trypanosome in-situ tagging vector

    Tags

    triple HA

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pMOTag53M

    Plasmid

    #26297

    Depositor

    George Cross

    Type

    Empty backbone

    Use

    Trypanosome in-situ tagging vector

    Tags

    triple cMyc

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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