We narrowed to 8,590 results for: dykddddk
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Plasmid#124299PurposeLentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertUBE2G1 (UBE2G1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85
Plasmid#111736PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ85 (polyQ repeat)PromoterCMV and P10Available SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-bsd-Flag-Trl1
Plasmid#126609PurposeExpresses N-terminal Flag-tagged yeast Trl1DepositorAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-E31A
Plasmid#125843Purposeexpress mutant BAP1-E31ADepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-S10T
Plasmid#125842Purposeexpress mutant BAP1-S10TDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG membrane-bound lipin-1 (TMD)
Plasmid#120277PurposeExpression of ER/Golgi-tethered lipin-1 in mammalian cellsDepositorInsertLipin-1 (Lpin1 Mouse)
TagsFLAG and in-frame fusion with the TMD Golgi-local…ExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR
Plasmid#113162PurposeA donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable markerDepositorInsertSpCas9
TagsFLAGExpressionMammalianMutationD10APromoterCAGAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD-MHC
Plasmid#113957Purposemammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class IDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationresidues 22-568 fused to a transmembrane tetherPromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113688PurposeSaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113689PurposeSaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACSDepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-FLAG
Plasmid#119233PurposePlasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.DepositorAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN3
Plasmid#110637PurposeRetroviral expression of Flag-SFXN3 in mammalian cellsDepositorAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN5
Plasmid#110639PurposeRetroviral expression of Flag-SFXN5 in mammalian cellsDepositorAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-mKO2
Plasmid#110371PurposeMammalian expression of mKO2 with FLAG tag, Flp-In T-Rex systemDepositorInsertmonomeric Kusabira Orange 2
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73
Plasmid#111724PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ73 (polyQ repeat)PromoterCMV and P10Available SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagB(GD)/RagC(CTD) chimera
Plasmid#112773Purposeexpress Flag-RagB (Gdomain) fused with RagC (C terminal domain)DepositorInsertFlag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)
TagsFlagExpressionMammalianAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CelTOS-COMP-blac-flag-his
Plasmid#111014PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertcell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
GRP94-COMP-blac-flag-his
Plasmid#111008PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertendoplasmin, putative (GRP94) (PFL1070c Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta2-PAmcherry1
Plasmid#114190Purposestudying clustering of Beta2 receptors by superresolution microscopy (PALM)DepositorInsertAdrenergic receptor beta 2 (ADRB2 Human)
Tags3HA, FLAG, and PAmcherry1ExpressionMammalianPromoterSV40Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAEBL-COMP-blac-flag-his
Plasmid#110963PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertmerozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only