We narrowed to 9,921 results for: pET
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Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109E (TAC-->TAT)
Plasmid#105735PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109E (TAC-->TAT) (SNCA Human)
ExpressionBacterialMutationQ109EPromoterT7Available sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
TagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET11a asyn Q109N (TAC-->TAT)
Plasmid#105737PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109N (TAC-->TAT) (SNCA Human)
ExpressionBacterialMutationQ109NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q99E (TAC-->TAT)
Plasmid#105734PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79E (TAC-->TAT)
Plasmid#105732PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-932)
Plasmid#73748PurposeExpression of GFP-PP-RPN2 (918-932) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-932 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (797-953)
Plasmid#74228Purpose[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
delNC C-His Ana GvpC in pET28a
Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
TagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
Plasmid#61943PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coliDepositorInsertsTags6x HISExpressionBacterialMutationResidues 2-39 deleted resulting in construct star…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16 M40R;W41H;H42S
Plasmid#45125DepositorInsertDnvLB16 M40R;W41H;H42S
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastMutationM40R;W41H;H42S to DnvLB16PromoterGALAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(166-611)
Plasmid#39338DepositorInsertElongation factor 1 alpha-like protein
TagsHis-tagExpressionBacterialPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p140 pETF-GST RanBP10 L301G
Plasmid#11093DepositorAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184741Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only