We narrowed to 6,988 results for: ef1a

1. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR V5-RNF14 C417A_IRES-mCherry

   Plasmid

   #198385

   Purpose

   RNF14 C417A expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RNF14 (RNF14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   catalytic cysteine mutation (C417A)

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR V5-RNF25 dRING_IRES-mCherry

   Plasmid

   #198387

   Purpose

   RNF25 (?RING) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RNF25 (RNF25 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   RING deletion (135-201)

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p101_CRISPRai_PiggyBac

   Plasmid

   #213777

   Purpose

   Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   VPR-dSaCas9
   dSpCas9-KRAB-BFP
   zeocin
   Tet-On transactivator
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a (EF-1-alpha intron a) and Tet_On_TRE

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lenti-NLS-mTagBFP2

   Plasmid

   #216128

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJL056

   Plasmid

   #177168

   Purpose

   Expresses wildtype MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

   Plasmid

   #125696

   Purpose

   Global labeling of inhibitory synapses (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   mRuby2-Gephyrin.FingR-IL2RGTC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge