We narrowed to 70,757 results for: LAS
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Plasmid#111255PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptideDepositorInsertTorT signal peptide
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT9993-Hypa-ABE
Plasmid#163001Purposeplasmid expressing Hypa-ABEDepositorInsertHypa-ABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9676-ABE
Plasmid#162997Purposeplasmid expressing ABEDepositorInsertABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCUX1.1.0-gDNA
Plasmid#112434PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1DepositorInsertCUX1 (CUX1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shScg2-CWB
Plasmid#223659PurposeExpresses hM3D(Gq) and a shRNA targetting Scg2 in a Cre-dependent mannerDepositorInserthM3D(Gq)-mCherry and shScg2
UseAAV and RNAiTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB
Plasmid#223660PurposeExpresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent mannerDepositorInserthM3D(Gq)-mCherry and shLacZ
UseAAV and RNAiTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shVgf-CWB
Plasmid#223658PurposeExpresses hM3D(Gq) and a shRNA targetting VGF in a Cre-dependent mannerDepositorInserthM3D(Gq)-mCherry and shVGF
UseAAV and RNAiTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRTagsExpressionBacterialMutationPromoterTetR-PT5-3Available sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRTagsExpressionBacterialMutationPromoterP3 constitutive promoter and native promoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Esyn-FlpX-TVA950-EYFP-WPRE
Plasmid#234710PurposepAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFPDepositorInsertTVA950-eYFP
UseAAV; Has fdio cassette for flp-dependent expressi…TagsExpressionMammalianMutationPromoterenhanced SynapsinAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2
Plasmid#231904PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121-Split-TALED
Plasmid#218171PurposeExpress plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA proteinDepositorInsertTALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121-mTCBE
Plasmid#218172PurposeExpress plastid monomeric TALE-linked CBE, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a human APOBEC3A variant (hA3A-Y130F), and UGIDepositorInsertTALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA332
Plasmid#214744PurposeLow copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA006
Plasmid#216056PurposeFragmid fragment: (2A Selection) Blasticidin resistanceDepositorHas ServiceCloning Grade DNAInsertT2A_v1.1; BlastR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA069
Plasmid#215995PurposeFragmid fragment: (Selection) blasticidin resistanceDepositorHas ServiceCloning Grade DNAInsertStart; BlastR_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu
Plasmid#216422PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 Prg4 promoter-luciferase
Plasmid#195034PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-eGFP-ACAP1
Plasmid#203800PurposeAAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorInsertACAP1 (ACAP1 Human)
UseAAVTagseGFPExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB511-TP-BVRA
Plasmid#203480PurposeFor Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.DepositorInsertRattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-HsTNRC6Biso1_1379-1723del1535-1624_P
Plasmid#147204PurposeBacterial Expression of HsTNRC6Biso1_1379-1723del1535-1624DepositorInsertHsTNRC6Biso1_1379-1723del1535-1624 (TNRC6B Human)
UseTagsExpressionBacterialMutationone non silent mutation T1486A compared to the se…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsTNRC6Biso1 1-1218_L
Plasmid#146887PurposeMammalian Expression of HsTNRC6Biso1_1-1218DepositorInsertHsTNRC6Biso1_1-1218 (TNRC6B Human)
UseTagsExpressionMammalianMutation4 non silent mutations, S441G, H1088R, Q1106R, Q1…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsTNRC6Biso1_1218-1723_L
Plasmid#146888PurposeMammalian Expression of HsTNRC6Biso1_1218-1723DepositorInsertHsTNRC6Biso1_1218-1723 (TNRC6B Human)
UseTagsExpressionMammalianMutationone non silent mutation T1486A and a deletion of …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso2_J
Plasmid#146696PurposeMammalian Expression of HsNot1iso2DepositorInsertHsNot1iso2 (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ7-DadR/DadS-Pdadh-LacZ
Plasmid#191622PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadSDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8-CadR-Pcadh-LacZ
Plasmid#191623PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only