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Addgene

We narrowed to 70,757 results for: LAS

Showing: 6451 - 6480 of 70757 results
  1. pCUX1.1.0-gDNA

    Plasmid
    #112434
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1
    Depositor
    Insert
    CUX1 (CUX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 constitutive promoter and native promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. LVDP 2P.2

    Plasmid
    #231904
    Purpose
    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs
    Depositor
    Insert
    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    R203M in N protein
    Promoter
    CMV, EF-1a
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pYPQ121-Split-TALED

    Plasmid
    #218171
    Purpose
    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein
    Depositor
    Insert
    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    Use
    Plastid base editing
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQ121-mTCBE

    Plasmid
    #218172
    Purpose
    Express plastid monomeric TALE-linked CBE, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a human APOBEC3A variant (hA3A-Y130F), and UGI
    Depositor
    Insert
    TALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI
    Use
    Plastid base editing
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid
    #216421
    Purpose
    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Tags
    3xmyc
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pPbU6_hdhfr/yfcu

    Plasmid
    #216422
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. AAV-sCAG-eGFP-ACAP1

    Plasmid
    #203800
    Purpose
    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pGWB511-TP-BVRA

    Plasmid
    #203480
    Purpose
    For Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.
    Depositor
    Insert
    Rattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pTP-PCR C1

    Plasmid
    #189618
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pTP-PCR C4

    Plasmid
    #189621
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, and minor additional mutat…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pTP-PCR C2

    Plasmid
    #189619
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 6451 - 6480 of 70757 results