We narrowed to 22,830 results for: Sis

1. Flag-Gsdmd-NT 4A

   Plasmid

   #80952

   Purpose

   Expresses Gsdmd-NT 4A mutant in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-NT 4A (Gsdmd Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-GFP1-10-pDEST-C

   Plasmid

   #118369

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-GFP1-10

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCVhygro-F-Ezh2

   Plasmid

   #24926

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-FLAG-UFM1

   Plasmid

   #134640

   Purpose

   Expression of FLAG-tagged human UFM1 wildtype

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 (UFM1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Halo-53BP1 HRD

   Plasmid

   #207087

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211622

   Purpose

   Rhno1 S141A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S141A

   Promoter

   CMV

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAL101

   Plasmid

   #22474

   Purpose

   Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PIF6APB
   

   Use

   Synthetic Biology

   Tags

   mYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHJ421

   Plasmid

   #20680

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Ezrin (EZR Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2HA-pDEST-C

    Plasmid

    #118374

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30b_OmpAT7Sav

    Plasmid

    #138589

    Purpose

    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

    Insert

    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    

    Use

    Synthetic Biology

    Tags

    OmpA export signal and T7 tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. UAS:Zebrabow-B

    Plasmid

    #118216

    Purpose

    For broad gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Alex Schier, Albert Pan

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SFG.CNb30_opt.IRES.eGFP

    Plasmid

    #22493

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)
    

    Use

    Retroviral

    Mutation

    L124T; K125-LA-Ins

    Available Since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. Halo-MDC1

    Plasmid

    #207108

    Purpose

    pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 in human cells.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag-MDC1
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. BiP-mCherry

    Plasmid

    #62233

    Purpose

    Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mCherry-KDEL
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB6633

    Plasmid

    #106162

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMaCTag-P23

    Plasmid

    #120034

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-Cer-NCreIntN

    Plasmid

    #160505

    Purpose

    Expresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCry2PHR-mCh-LRP6c

    Plasmid

    #42960

    Purpose

    Expresses optogenetically controlled LRP6 (C-terminal domain)

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    CMV

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. Lenti-DAG1

    Plasmid

    #205149

    Purpose

    Overexpress DAG1

    Depositor

    Monkol Lek

    Insert

    Dystroglycan 1 (DAG1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Carries common missense variant c.41C>G (p.Ser…

    Promoter

    EF-1a

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLD1 translation factors

    Plasmid

    #117760

    Purpose

    BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 1-13
    

    Use

    Escherichia coli

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMaCTag-P29

    Plasmid

    #120040

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK7-FLAG-TSC2

    Plasmid

    #8996

    Depositor

    John Blenis

    Article

    Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    TSC2 (TSC2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG416GPD-Cerulean-VPS21

    Plasmid

    #18842

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    VPS21 (VPS21 Budding Yeast)
    

    Tags

    Cerulean

    Expression

    Yeast

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA Flag-Smad4M

    Plasmid

    #14959

    Depositor

    Joan Massague

    Article

    Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

    Insert

    Smad4 (SMAD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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28. pJL33

    Plasmid

    #227430

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    mWasabi
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJL34

    Plasmid

    #227431

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLD3 translation factors

    Plasmid

    #117762

    Purpose

    BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 22-30
    

    Use

    Escherichia coli

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPB-cT3G-cERP2-HMGB2

    Plasmid

    #222532

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HMGB2 (HMGB2 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLD2 translation factors

    Plasmid

    #117761

    Purpose

    BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 14-21
    

    Use

    Escherichia coli

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ATM-Halo HRD

    Plasmid

    #207082

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    None

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CAV1-P132L-mEGFP

    Plasmid

    #27708

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    P132L

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LZRS-hNEDD9_IresGFP

    Plasmid

    #21962

    Depositor

    Lynda Chin

    Article

    Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

    Insert

    NEDD9 (NEDD9 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

36. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Promoter

    hPGK

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBI121-GFP-RmMYB108

    Plasmid

    #173181

    Purpose

    Expresses Rosa multiflora MYB108 for subcellular localization in plants

    Depositor

    Daidi Che

    Article

    Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

    Insert

    RmMYB108
    

    Expression

    Plant

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

    Plasmid

    #233539

    Purpose

    A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

    Plasmid

    #233541

    Purpose

    A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-cortactin

    Plasmid

    #26722

    Depositor

    Anna Huttenlocher

    Article

    Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

    Insert

    Cortactin (Cttn Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge