We narrowed to 7,286 results for: 11

1. pNCST-AvicFP1

   Plasmid

   #129504

   Purpose

   Expresses AvicFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP1
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCST-AvicFP4

   Plasmid

   #129507

   Purpose

   Expresses AvicFP4 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP4
   

   Expression

   Bacterial

   Mutation

   One of two variants of AvicFP4 tested with essent…

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-GRAB_rDA1m

   Plasmid

   #140556

   Purpose

   Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA sensor GRAB_rDA1m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCST-AvicFP2

   Plasmid

   #129505

   Purpose

   Expresses AvicFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP2
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCST-AvicFP3

   Plasmid

   #129506

   Purpose

   Expresses AvicFP3 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP3
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Rubicon

   Plasmid

   #21636

   Depositor

   Tamotsu Yoshimori

   Article

   Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

   Insert

   Rubicon (1700021K19Rik Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   *(see below)

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-DsRed2

   Plasmid

   #112219

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   DsRed2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   DsRed2 contains a silent nucleotide substitution …

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1.Gα12-LgB115

   Plasmid

   #134363

   Purpose

   Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα12-LgB115 (GNA12 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB1-A11(2-196, G38R)-Strep

    Plasmid

    #196209

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G38R

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSIIpuro-TGFa-ScNeo

    Plasmid

    #209895

    Purpose

    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-ScNeo (TGFA Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1.D2-NP

    Plasmid

    #134366

    Purpose

    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    D2-NP (DRD2 Human)
    

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSIIpuro-NRG1-ScNeo

    Plasmid

    #209900

    Purpose

    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    NRG1-ScNeo (NRG1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZac2.1 U6-SaAi9L-U6-SaAi9R

    Plasmid

    #78604

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    gRNAs for SaCas9 targeting Ai9 locus
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pHR-Hsp-eGFP-PGK-mCherry

    Plasmid

    #191862

    Purpose

    Heat inducible eGFP reporter with constitutive mCherry marker

    Depositor

    Yingxiao Wang

    Article

    Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

    Inserts

    eGFP
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    HSPA7 promoter and PGK promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-GRAB_eCB2.0

    Plasmid

    #164606

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-SMS2-TEV-Twin-Strep

    Plasmid

    #202529

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS2 (SGMS2 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TGA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GB1-A11(2-196, G175R)-Strep

    Plasmid

    #196211

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G175R

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFR D770-N771 insNPG

    Plasmid

    #11016

    Purpose

    Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR D770-N771 insNPG (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    D770-N771 insNPG

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only