We narrowed to 4,353 results for: erf

1. pCMV_msfGFP-SEPT7 Gmut1

   Plasmid

   #180316

   Purpose

   mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT7 W269A, H279D

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_SEPT9_i3 NCmut-msfGFP

   Plasmid

   #180325

   Purpose

   mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 I263D, M270D

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

   Plasmid

   #136688

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Use

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression w/ three mut…

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23 C5_12

   Plasmid

   #60319

   Purpose

   This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   Non active element (nearest TSS C18orf26)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGL4.23 C5_14

   Plasmid

   #60321

   Purpose

   This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   Non active element (nearest TSS CCRN4L)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. CXCR4-SZ158a QTY

   Plasmid

   #162442

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-X-C chemokine receptor type 4 (CXCR4 Human)
   

   Use

   Tags

   his-tag, Rho-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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7. CXCR4-SZ146a QTY

   Plasmid

   #162443

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-X-C chemokine receptor type 4 (CXCR4 Human)
   

   Use

   Tags

   his-tag, Rho-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234454

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234441

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Has Service

   AAV1

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234442

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Has Service

    AAV1

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234453

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234448

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid

    #192786

    Purpose

    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNGR1 (IFNGR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSems-leader-mXFPe-IFNGR2(30-337)

    Plasmid

    #192787

    Purpose

    Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNGR2 (IFNGR2 Human)
    

    Use

    Tags

    Ig k-chain leader sequence and mXFPe

    Expression

    Mammalian

    Mutation

    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid

    #185448

    Purpose

    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    See Depositor Comments Below

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AICSDP-45: TTN-mEGFP

    Plasmid

    #114412

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234447

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234440

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid

    #231548

    Purpose

    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid

    #208035

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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