We narrowed to 42,575 results for: cha

1. pBiT3.1-N_RBBP4:1-425

   Plasmid

   #211037

   Purpose

   Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav2.2 W391A pcDNA3

   Plasmid

   #206085

   Purpose

   expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

   Depositor

   Annette Dolphin

   Article

   Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

   Plasmid

   #106469

   Purpose

   Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(S219,D415,H474A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Serine 219, Aspartate 415 and Histidine 4…

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CYLBL_Neo_CAGdCas9-KRAB

   Plasmid

   #220439

   Purpose

   CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

   Depositor

   Yin Shen

   Article

   Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

   Insert

   dCas9
   

   Use

   CRISPR and TALEN

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

   Plasmid

   #91819

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNLF1-C_PLCG1:1-1291

   Plasmid

   #211089

   Purpose

   Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   PLCG1:M1-L1291 (PLCG1 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   isoform 2. I813T natural variant (VAR_011908)

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBiT3.1-N_FBXW7:1-707

   Plasmid

   #211099

   Purpose

   Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW7:M1-K707 (FBXW7 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBiT3.1-C_FBXW7:1-707

   Plasmid

   #211104

   Purpose

   Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW7:M1-K707 (FBXW7 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNLF1-N_CCT5:1-541

   Plasmid

   #210978

   Purpose

   Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CCT5:M1-E541 (CCT5 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNLF1-N_FBXW7:1-707

    Plasmid

    #210946

    Purpose

    Mammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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