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pCL417_PB_miRE_tre_3xFLAG_mHNRNPK

Plasmid

#251826

Purpose

Doxycycline-inducible 3xFLAG-tagged mouse HNRNPK

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

Heterogeneous nuclear ribonucleoprotein K (Hnrnpk )

Expression

Mammalian

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-YTHDC1_sgRNA_4

Plasmid

#242893

Purpose

PiggyBac cargo vector with YTHDC1 sgRNA 4 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

YTHDC1 (Ythdc1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-YTHDC1_sgRNA_2a

Plasmid

#242894

Purpose

PiggyBac cargo vector with YTHDC1 sgRNA 2a for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

YTHDC1 (Ythdc1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac1-eIF3c-3C-STREP

Plasmid

#240026

Purpose

Human eIF3c for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3c (EIF3C Human)

Tags

3C_STREP II

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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mCerulean-T-SpyTag

Plasmid

#243787

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCerulean from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCerulean

Tags

6x His tag

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGF-A-SpyTag

Plasmid

#246944

Purpose

Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199

Insert

Please see depositor comments below

Tags

6x His tag

Expression

Bacterial

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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bla-A OR T-SpyTag

Plasmid

#246945

Purpose

Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199

Insert

Please see depositor comments below

Tags

6x His tag

Expression

Bacterial

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag

Plasmid

#240484

Purpose

Express engineered glycosylated eGFP in CHO cells

Depositor

Xi Ren

Article

Xing et al Acta Biomater. 2025 Nov;207:221-232. doi

Insert

glycosylated eGFP

Tags

3 repeats of hVEGF165 glycosylation sequon, 6xHis…

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-S-SpyTag

Plasmid

#243786

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCherry

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TVMV Protease-mCherry

Plasmid

#243794

Purpose

Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A-SpyTag

Plasmid

#243769

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S-SpyTag

Plasmid

#243770

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T-SpyTag

Plasmid

#243771

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR S-SpyTag

Plasmid

#243772

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR T-SpyTag

Plasmid

#243773

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mGreenLantern-S OR T-SpyTag

Plasmid

#243774

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mGreenLantern-A AND T-SpyTag

Plasmid

#243776

Purpose

Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-PIPKγ87-D253A

Plasmid

#242825

Purpose

Encodes human PIPKγ87-D253A and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Expression

Mammalian

Mutation

D253A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-PIPKγ87

Plasmid

#242824

Purpose

Encodes wildtype human PIPKγ87 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP

Plasmid

#240128

Purpose

Expresses GFP in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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