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pAAV-CMV-intron-CasRx-pA

Plasmid

#192487

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_N300

Plasmid

#228814

Purpose

Expression of human EHMT1 N-terminal 300 aa only

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1-300 only

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_Ank+SET

Plasmid

#228815

Purpose

Expression of human EHMT1 with deletion of N-terminal 300 aa

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of N-terminal 300 aa

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-EGFP-WPRE-ID-PolyA

Plasmid

#241829

Purpose

AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

EGFP-WPRE-ID-PolyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p53-G609T

Plasmid

#229531

Purpose

Expresses p53 mutant CASM203 in mamallian cells

Depositor

Robin Fåhraeus

Article

Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

Insert

p53-CDS (TP53 Human)

Expression

Mammalian

Mutation

c.G609T

Promoter

CMV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p53-G609A

Plasmid

#229532

Purpose

Expresses p53 mutant G609A in mamallian cells

Depositor

Robin Fåhraeus

Article

Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

Insert

p53-CDS (TP53 Human)

Expression

Mammalian

Mutation

c.G609A

Promoter

CMV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p53-G609C

Plasmid

#229533

Purpose

Expresses p53 mutant G609C in mamallian cells

Depositor

Robin Fåhraeus

Article

Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

Insert

p53-CDS (TP53 Human)

Expression

Mammalian

Mutation

c.G609C

Promoter

CMV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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ER-frNFAST

Plasmid

#233599

Purpose

Expression of frNFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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PM-frNFAST

Plasmid

#233602

Purpose

Expression of frNFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1B1-3

Plasmid

#228811

Purpose

Expression of mouse EHMT1 with deletion of aa 1185-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1185-1298

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1B1-4

Plasmid

#228812

Purpose

Expression of mouse EHMT1 with deletion of aa 809-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 809-1298, See Depositor Comments

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MfnG-EcYRS-EGFP*

Plasmid

#191119

Purpose

A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein

Tags

His tag

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Tags

FLAG and His

Expression

Bacterial

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

Plasmid

#221082

Purpose

Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2

Use

Synthetic Biology

Promoter

CMV, T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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