We narrowed to 470 results for: plasmid cloning by pcr
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Plasmid#234651PurposeThis plasmid encodes a mutated version of the genetically encoded chemokine biosensor LoX3-1.0, which is designed to be non-responsive to chemokine binding.DepositorInsertGPCR activation-based mutant chemokine CXCL9-11 sensor GRAB_LoX3-mut
ExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OT1.0
Plasmid#185387PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVPromoterEFSAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVPromoterEFSAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PuroR-P2A-DD-TCOF1-donor
Plasmid#60747PurposeDonor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
BlastR-P2A-DD-TCOF1-donor
Plasmid#60748PurposeDonor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1h
UseAAVPromoterhSynAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVPromoterhSynAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GACh2.0
Plasmid#121921Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0DepositorInsertGPCR activation based ACh sensor GACh2.0
UseAAVPromoterhSynAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
hNTo1-qgRNA-pYJA5
Plasmid#217781PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNTo2-qgRNA-pYJA5
Plasmid#217782PurposeNon-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNTa1-qgRNA-pYJA5
Plasmid#217779PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNTa2-qgRNA-pYJA5
Plasmid#217780PurposeNon-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3h
Plasmid#208699PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3h
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3m
Plasmid#208702PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3h
Plasmid#208703PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseAAVPromoterhSynAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1m
Plasmid#123308PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1m
UseAAVMutationcontains a glycine-to-threonine mutation at posit…PromoterhSynAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA2m
Plasmid#208700PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA2m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA2h
Plasmid#208701PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA2h
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3m
Plasmid#208698PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neuronsDepositorHas ServiceAAV1InsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3mut
Plasmid#208704PurposeExpresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_gDA3mut in neuronsDepositorInsertGPCR activation based dopamine (DA) control sensor GRAB_gDA3mut
UseAAVPromoterhSynAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only