We narrowed to 23,178 results for: Nov

1. pcDNA3_2FLAG-AKAP79ΔCN

   Plasmid

   #183451

   Purpose

   Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   aa337-343 deleted

   Promoter

   CMV

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTC223

   Plasmid

   #70019

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-hPREP

   Plasmid

   #179387

   Purpose

   Express human prolyl oligopeptidase (tagged) in bacterial cells

   Depositor

   Christian Gruber

   Article

   Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

   Insert

   human prolyl oligopeptidase (PREP Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-CD81-(TEV)-Cre

   Plasmid

   #167939

   Purpose

   human CD81-TEV cleavage site-Cre

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD81 (CD81 Human)
   

   Use

   Tags

   Cre recombinase and TEV protease cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mc2 mNeon EF1a

   Plasmid

   #206224

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK1035

   Plasmid

   #8832

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

   Insert

   TVMV protease
   

   Use

   Tags

   His and MBP (cleaved)

   Expression

   Bacterial

   Mutation

   synthetic gene with codons optimized for E. coli

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFosill-3

   Plasmid

   #89697

   Purpose

   to make Fosmids that can be converted into Illumina sequencable libraries

   Depositor

   Andreas Gnirke

   Article

   Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. N-terminal Halo-XLF

   Plasmid

   #207546

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TrpLE-M-nMSP1E3D1

    Plasmid

    #179949

    Purpose

    Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

    Depositor

    Kerney Glover

    Article

    Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

    Insert

    TrpLE-M-nMSP1E3D1
    

    Use

    Tags

    TrpLE

    Expression

    Bacterial

    Mutation

    Native Trp residues have been mutated to Phe resi…

    Promoter

    T7

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL90003

    Plasmid

    #68262

    Purpose

    Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6 (Triticum aestivum)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-scrCdh20.1 GFP

    Plasmid

    #209106

    Purpose

    GFP expressing scramble of shRNA targeting Cdh20

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCdh20.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Expression

    Mutation

    Promoter

    FCP and U6

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFosill-2

    Plasmid

    #89696

    Purpose

    to make Fosmids that can be converted into Illumina sequencable libraries

    Depositor

    Andreas Gnirke

    Article

    Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmScarlet_NES_C1

    Plasmid

    #85060

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCBC-MT3T4

    Plasmid

    #50595

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFosill-4

    Plasmid

    #89698

    Purpose

    to make Fosmids that can be converted into Illumina sequencable libraries

    Depositor

    Andreas Gnirke

    Article

    Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ePB-PURO-TT-PA-CRE

    Plasmid

    #179584

    Purpose

    ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    PA-MAG-CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-Cx35-Halo-C-end

    Plasmid

    #71512

    Purpose

    Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

    Depositor

    John O'Brien

    Article

    Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

    Insert

    Connexin 35
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge