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pUPD2_5x 2EBS-S10 (A2)

Plasmid

#254058

Purpose

Contains synthetic promoter 5x 2EBS-S10

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2024 Jul;22(7):1942-

Insert

5x 2EBS-S10 (A2)

Use

Synthetic Biology

Expression

Plant

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD2_5x 2DR5-2DR5v2 (A1)

Plasmid

#254059

Purpose

Contains synthetic promoter 5x 2DR5-2DR5v2

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2024 Jul;22(7):1942-

Insert

5x 2DR5-2DR5v2 (A1)

Use

Synthetic Biology

Expression

Plant

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_LS-R1

Plasmid

#254022

Purpose

A plasmid containing the LS-R1 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

LS-spacer-R1

Use

Synthetic Biology

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L2-RE

Plasmid

#254025

Purpose

A plasmid containing the L2-RE connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L2-spacer-RE

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L7-R8

Plasmid

#254031

Purpose

A plasmid containing the L7-R8 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L7-spacer-R8

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L2-R3

Plasmid

#254024

Purpose

A plasmid containing the L2-R3 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L2-spacer-R3

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L5-R6

Plasmid

#254029

Purpose

A plasmid containing the L5-R6 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L5-spacer-R6

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L1-R2

Plasmid

#254023

Purpose

A plasmid containing the L1-R2 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L1-spacer-R2

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L8-RE

Plasmid

#254033

Purpose

A plasmid containing the L8-RE connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L8-spacer-RE

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L3-R4

Plasmid

#254026

Purpose

A plasmid containing the L3-R4 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L3-spacer-R4

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L4-R5

Plasmid

#254027

Purpose

A plasmid containing the L4-R5 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L4-spacer-R5

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2498

Plasmid

#252581

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA6 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtCHLl1 gRNA6 and gRNA 4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2652

Plasmid

#252582

Purpose

TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtCHLl1 gRNA4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2653

Plasmid

#252583

Purpose

TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA6

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtCHLl1 gRNA6

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2660

Plasmid

#252584

Purpose

TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA9

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtCHLl1 gRNA9

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVS-RT-pDGB3alpha2

Plasmid

#240730

Purpose

BCTV vector containing the GUS:NPTII repari template. Lacks the BCTV virion sense ORFs.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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BCTV-VS domesticated-pUPD2

Plasmid

#240748

Purpose

Domesticated BCTV virion sense ORFs with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Mutation

2nt substitution in V3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-pUPD2

Plasmid

#240715

Purpose

BCTV complementary sense ORFs with mutated Rep with GoldenBraid C1 grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTV-VS WT-pUPD2

Plasmid

#240716

Purpose

WT BCTV virion sense ORFs prior to domestication for GoldenGate cloning with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVS-YPET-pDGB3alpha1

Plasmid

#240723

Purpose

BCTV vector for YPET expression. Lacks the BCTV virion sense ORFs.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

YPET

Expression

Plant

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-YPET-pDGB3alpha1

Plasmid

#240725

Purpose

BCTV vector for YPET expression. Rep has been mutated.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

YPET

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-RT-pDGB3alpha1

Plasmid

#240728

Purpose

BCTV vector containing the GUS:NPTII repair template for HDR experiments. Rep has been mutated.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_HyPB_RFP

Plasmid

#248201

Purpose

HyPB RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_Antgra4_RFP

Plasmid

#248203

Purpose

Antgra4 RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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excision_reporter_HyPB

Plasmid

#248200

Purpose

HyPB and synthetic transposase excision reporter and targeted integration reporter

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

Excision RFP reporter

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pXD80EN7-Pct3-(–)-Eldh2(cHis6)-noAccs(ElA2)

Plasmid

#233878

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF24-Pct3-GsVadh(nHis6)(Gs28C)

Plasmid

#233872

Purpose

Expresses His-tagged Gordonibacter sp. 28C Gs Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Gs Vadh

Tags

His

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN15-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225327

Purpose

Expresses Eggerthella lenta A2 Dadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0053_pIVTRup_EBFP2

Plasmid

#242544

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EBFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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